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. 2025 May 6:16:1580705.
doi: 10.3389/fphar.2025.1580705. eCollection 2025.

Adjuvant treatment with Wu-Zi-Yan-Zong formula for abnormal sperm parameters associated with male infertility: a meta-analysis of randomized controlled trials

Feilun Cui #  1   2 , Yueshi Zhang #  3 , Yu Fan  4
Affiliations

Adjuvant treatment with Wu-Zi-Yan-Zong formula for abnormal sperm parameters associated with male infertility: a meta-analysis of randomized controlled trials

Feilun Cui et al. Front Pharmacol. .

Abstract

Background: Wu-Zi-Yan-Zong (WZYZ) formula is a traditional Chinese botanical drug that has been used to treat male infertility. This meta-analysis aims to evaluate its effectiveness of the WZYZ formula as an adjuvant therapy for treating abnormal sperm parameters associated with male infertility.

Methods: A comprehensive literature search was conducted using PubMed, Embase, Cochrane Library, Web of Science, SinoMed, Wanfang, and CNKI databases until December 12, 2024 to identify randomized controlled trials (RCTs) that assessed the effectiveness of the WZYZ formula as an adjuvant therapy for treating abnormal sperm parameters associated in men with infertility. For dichotomous data, the pooled results were summarized as risk ratio (RR) with 95% confidence intervals (CI), while continuous data were expressed as pooled weighted mean difference (WMD) with 95% CI.

Results: A total of 11 RCTs involving 951 men were identified. The pooled results showed that the WZYZ formula, when combined with control treatment, significantly improved the pregnancy rate of female partners (RR 1.68; 95% CI 1.34-2.11), semen volume (WMD 0.58 mL; 95% CI 0.28-0.89), sperm concentration (WMD 6.87 × 106/mL; 95%CI 4.24-9.51), total sperm motility (WMD 15.55%; 95% CI 10.38-20.72), forward grade (a) sperm motility (WMD 5.44%; 95% CI 1.86-9.01), forward grade (a + b) sperm motility (WMD 7.14%; 95% CI 4.04-10.23), abnormal sperm morphology (WMD -10.38%; 95% CI -15.72 to -5.03), and activity of the acrosome enzyme (WMD 8.02 × 106 μIU; 95% CI 3.58-12.46.

Conclusion: Adjuvant treatment with WZYZ formula significantly improves the pregnancy rate of female partners by improving several semen parameters in infertile men with abnormal sperm parameters. However, further well-designed RCTs with larger sample sizes are necessary to definitively determine the efficacy and safety of the WZYZ formula in treating abnormal sperm parameters associated with male infertility.

Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/view/CRD42024629510.

Keywords: Wu-Zi-Yan-Zong formula; abnormal sperm parameters; male infertility; meta-analysis; randomized controlled trials.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Flow chart of the trial selection process.
FIGURE 2
FIGURE 2
Risk of bias graph (A) and Risk of bias summary (B).
FIGURE 3
FIGURE 3
Pooled RR with 95% CI for pregnancy rates of female partners compared with or without Wu-Zi-Yan-Zong formula treatment.
FIGURE 4
FIGURE 4
Pooled WMD with 95% CI for sperm concentration compared with or without Wu-Zi-Yan-Zong formula treatment.
FIGURE 5
FIGURE 5
Pooled WMD with 95% CI for sperm motility compared with or without Wu-Zi-Yan-Zong formula treatment.
FIGURE 6
FIGURE 6
Pooled WMD with 95% CI for forward grade (a) sperm motility (A) and forward grade (a + b) sperm motility (B) compared with or without Wu-Zi-Yan-Zong formula treatment.
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