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. 2025 May 21;52(1):474.
doi: 10.1007/s11033-025-10552-w.

The MNS blood group system in Tunisian blood donors: common and rare alleles

Mohamed Hichem Sellami  1 , Eya Ghazouani  2 , Sondess Hadj Fredj  3 , Hamida Ferchichi  2 , Manel Chaabane  2 , Houda Kaabi  2 , Taieb Messaoud  3 , Slama Hmida  2
Affiliations

The MNS blood group system in Tunisian blood donors: common and rare alleles

Mohamed Hichem Sellami et al. Mol Biol Rep. .

Abstract

Background: The MNS blood group system is highly complex and characterized by the presence of major polymorphic antigens, namely MNS1/MNS2 and MNS3/MNS4. This study aims to examine the genetic basis of the common MNS antigens in Tunisian blood donors and discussed their importance in transfusion immunology.

Methods and results: A group of 229 Tunisian blood donors were genotyped for the major alleles GYPA*01, GYPA*02, GYPB*03 and GYPB*04 using the SSP-PCR method. A specific GYPA region was sequenced in some subjects to examine if there is a rare allele. The statistical analysis was done using the HaploView Software. The molecular analysis showed that 4 blood donors are probably carrier of a rare GYPA allele. Furthermore, it appeared that the GYPA*01 and GYPB*04 alleles; and the GYPA*01-GYPB*04 haplotype are predominant in this series. As for the phenotype, the study showed that the MNS:1,2,-3,4 type is the most frequent in recruited blood donors.

Conclusions: Overall, the study showed a balanced distribution of the targeted alleles. Furthermore, the observed frequencies appeared to be in harmony with that reported in other populations. Considering the importance of these findings, we think that this work will be an important contribution to the field of public health and epidemiology.

Keywords: Alloimmunization; Blood; MNS antigens; PCR-SSP; Tunisians.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: Written informed consent was signed by all subjects who have been recruited to participate in this study. The protocol was approved by the scientific committee of the National Blood Transfusion Centre of Tunis (Tunisia) Competing interests: The authors declare no competing interests.

References

    1. The International Society of Blood Transfusion (ISBT) (2025) Table of blood group antigens within systems. Available online at: https://www.isbtweb.org/resource/tableofbloodgroupantigenswithinsystems.... . Accessed 22 March
    1. Gassner C, Denomme GA, Portmann C et al (2020) Two prevalent ∼100-kb GYPB deletions causative of the GPB-Deficient blood group MNS phenotype S-s-U- in black Africans. Transfus Med Hemother 47:326–336. https://doi.org/10.1159/000504946 - DOI - PubMed - PMC
    1. Christensen RD, Bahr TM, Ilstrup SJ, Dizon-Townson DS (2023) Alloimmune hemolytic disease of the fetus and newborn: genetics, structure, and function of the commonly involved erythrocyte antigens. J Perinatol 43:1459–1467. https://doi.org/10.1038/s41372-023-01785-3 - DOI - PubMed
    1. Karim S (2013) Antibody identification. In: Shaz BH, Hillyer CD, Roshal M, Abrams CS (eds) Transfusion medicine and hemostasis clinical and laboratory aspects. Elsevier, USA, pp 127–136
    1. Peyrard T (2015) Système MNS (ISBT 002); in: Pascal Bailly, Jacques Chiaroni, Francis Roubinet (Eds.). Les groupes sanguins érythrocytaires, France, John Libbey Eurotext, pp. 35–49

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