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. 2025 Apr 24;11(1):dvaf010.
doi: 10.1093/eep/dvaf010. eCollection 2025.

Prenatal per- and polyfluoroalkyl substance exposures and DNA methylation among newborns in the Environmental influences on Child Health Outcomes program

Rose Schrott  1 , Christine Ladd-Acosta  1 , Vasantha Padmanabhan  2 , Dana Boyd Barr  3 , Carrie V Breton  4 , Andres Cardenas  5 , Courtney C Carignan  6 , Dana Dabelea  7 , Anne L Dunlop  8 , Danielle M Fallin  9 , Marie-France Hivert  10 , Ellen M Howerton  1 , Anna K Knight  8 , Emily Oken  10 , Alicia K Peterson  11 , Michael C Petriello  12 , Douglas Ruden  13 , Rebecca J Schmidt  14 , Alicia K Smith  8 , Anne P Starling  7   15 , Ivana V Yang  16 , Yeyi Zhu  11 , Jaclyn M Goodrich  17
Affiliations

Prenatal per- and polyfluoroalkyl substance exposures and DNA methylation among newborns in the Environmental influences on Child Health Outcomes program

Rose Schrott et al. Environ Epigenet. .

Abstract

Gestation is a vulnerable window when exposure to per- and polyfluoroalkyl substances (PFAS) may impact child development and health. Epigenetic modification, including DNA methylation (DNAm), may be one mechanism linking prenatal PFAS exposure to offspring outcomes. We tested associations between prenatal PFAS and newborn DNAm in 1017 participants from 6 cohorts in the US Environmental influences on Child Health Outcomes consortium. Concentrations of PFAS [perfluorooctanesulfonic acid (PFOS), perfluorooctanoic acid (PFOA), perfluorohexanesulfonic acid (PFHxS), perfluorononanoic acid (PFNA), and perfluorodecanoic acid] were measured in maternal serum or plasma. DNAm was quantified in newborn dried blood spot or umbilical cord blood leukocytes using the Infinium HumanMethylation450 (450K) or MethylationEPIC (EPIC) arrays. We tested associations between prenatal PFAS and neonatal blood DNAm on the 450K (n = 772) and EPIC (n = 245) arrays; results were meta-analysed across the platforms. Regional changes in DNAm were investigated, and findings were checked for replication in the Michigan Mother-Infant Pairs (MMIP) cohort (n = 140). Following correction for false discovery rate (q = 0.1 for meta-analyses), we identified an association between PFHxS and one cytosine-guanine (CpG) mapped to CASC3 (q = 0.065) that replicated in MMIP (P = .006). PFOS was associated with six CpG sites, of which five were mapped to the genes KIAA1841, ABR, LEP, SERPINA1, and LOXL1. One differentially methylated region (DMR) was associated with prenatal PFOA exposure, and one DMR was associated with PFOS exposure. In this multicohort analysis including a diverse group from the USA, PFOA, PFOS, PFHxS, and PFNA exposures in pregnancy were associated with offspring DNAm, and the implications for children's health merit further exploration.

Keywords: DNA methylation; children’s health; developmental origins of health and disease; epigenetics; epigenomics; per- and polyfluoroalkyl substances; perfluorinated chemicals.

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Conflict of interest statement

None declared.

Figures

Figure 1.
Figure 1.
Participants included in the analysis.
See this image and copyright information in PMC

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