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. 2025:2930:219-227.
doi: 10.1007/978-1-0716-4558-1_15.

Exploring the Cancer Immune Epigenome by ATAC-Seq

Chunling Zeng  1 , Yuexiang Wang  2
Affiliations

Exploring the Cancer Immune Epigenome by ATAC-Seq

Chunling Zeng et al. Methods Mol Biol. 2025.

Abstract

The Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is a rapid and sensitive method for exploring the cancer immune epigenome. ATAC-seq captures open chromatin sites based on transposing the sequencing adapter directly into native chromatin in vitro. Here we describe an optimized protocol for ATAC-seq with four main steps: nucleus extraction, transposition, library amplification, sequencing and data analysis. The ATAC protocol has the advantages of low sample requirements, high speed, and applicability to a wide range of cell and tissue types.

Keywords: ATAC-seq; Cancer immune epigenome; Open chromatin.

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