NAmiRNA-31 regulates cell cycle by activating ZWINT and promotes lung adenocarcinoma tumor development

Abstract

ObjectiveThis study aimed to identify Nuclear-activated miRNA (NAmiRNA) and their target genes linked to the prognosis of Lung adenocarcinoma tumor (LUAD), and provide novel diagnostic and therapeutic targets for this devastating disease.MethodsPutative target (Messenger RNA)mRNA of the prognostic (MicroRNA)miRNA was obtained by interesting the differentially expressed mRNAs with predicted targets from targetscan database. Functional validation experiments were conducted utilizing LUAD cell lines to corroborate the interactions between the identified miRNA and their target mRNAs. Collect cancer and adjacent tissues from patients to detect the expression of target genes. The effects of miRNA-target gene interactions on LUAD cell growth were investigated by (Cell Counting Kit-8)CCK8, colony formation assay and flow cytometry.ResultsOur findings revealed that both miR-31 and its target gene, ZWINT, were considerably overexpressed in LUAD individuals. The results of qPCR and WB indicated that miR-31 effectively upregulated the expression of (Zeste White 10 interactor)ZWINT. The transfection with miR-31mimic enhanced the activity of LUAD cells and promoted colony growth. Knockdown of ZWINT expression resulted in (G2 to mitosis)G2/M phase cell arrest, concurrent with a decrease in Cyclin B1 and (Cyclin-Dependent Kinase 1)CDK1 proteins.Conclusion(nuclear activating mirna-31)NAmiRNA-31 and ZWINT are upregulated in LUAD, suggest their potential as biomarkers for the unfavorable prognosis.

Keywords: NAmiRNA-31; ZWINT; cell cycle; cell proliferation; prognosis markers.