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. 1975 Sep;107(2):115-24.
doi: 10.1007/BF00446830.

Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica

P Rowell, W D Stewart

Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica

P Rowell et al. Arch Microbiol. 1975 Sep.

Abstract

The L-alanine dehydrogenase (ADH) of Anabaena cylindrica has been purified 700-fold. It has a molecular weight of approximately 270,000, has 6 sub-units, each of molecular weight approximately 43,000, and shows activity both in the aminating and deaminating directions. The enzyme is NADH/NAD+ specific and oxaloacetate can partially substitute for pyruvate. The Kampp for NAD+ is 14 muM and 60 muM at low and high NAD concentrations respectively.

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References

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