doi: 10.1007/BF00446830.
Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica
- PMID: 4043
- DOI: 10.1007/BF00446830
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Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica
Arch Microbiol.
1975 Sep.
Abstract
The L-alanine dehydrogenase (ADH) of Anabaena cylindrica has been purified 700-fold. It has a molecular weight of approximately 270,000, has 6 sub-units, each of molecular weight approximately 43,000, and shows activity both in the aminating and deaminating directions. The enzyme is NADH/NAD+ specific and oxaloacetate can partially substitute for pyruvate. The Kampp for NAD+ is 14 muM and 60 muM at low and high NAD concentrations respectively.
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