A simplified method for isocratic HPLC analysis of polyamines
- PMID: 4043337
- DOI: 10.1007/BF01951733
A simplified method for isocratic HPLC analysis of polyamines
Abstract
A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.
Similar articles
-
Chromophoric determination of putrescine, spermidine and spermine with dabsyl chloride by high-performance liquid chromatography and thin-layer chromatography.J Chromatogr. 1982 Feb 12;227(2):369-77. doi: 10.1016/s0378-4347(00)80390-4. J Chromatogr. 1982. PMID: 7061651
-
A dansyl chloride-HPLC method for the determination of polyamines.Methods Mol Biol. 1998;79:119-23. doi: 10.1385/0-89603-448-8:119. Methods Mol Biol. 1998. PMID: 9463826 No abstract available.
-
Determination of dansylated polyamines in red blood cells by liquid chromatography-tandem mass spectrometry.Anal Biochem. 2009 Jul 1;390(1):46-51. doi: 10.1016/j.ab.2009.04.007. Epub 2009 Apr 11. Anal Biochem. 2009. PMID: 19364488
-
Assay procedures for polyamines in urine, serum, and cerebrospinal fluid.Clin Chem. 1977 Sep;23(9):1519-26. Clin Chem. 1977. PMID: 330019 Review.
-
[Polyamine analysis of blood, tissue and gastric juice and its clinical significance. II. Analysis of tissue polyamines and their derivatives and their significance].Rinsho Byori. 1984 Jul;Spec No 59:167-75. Rinsho Byori. 1984. PMID: 6389934 Review. Japanese. No abstract available.
Cited by
-
Cerebral ischemia enhances polyamine oxidation: identification of enzymatically formed 3-aminopropanal as an endogenous mediator of neuronal and glial cell death.J Exp Med. 1998 Jul 20;188(2):327-40. doi: 10.1084/jem.188.2.327. J Exp Med. 1998. PMID: 9670045 Free PMC article.