A simplified method for isocratic HPLC analysis of polyamines
- PMID: 4043337
- DOI: 10.1007/BF01951733
A simplified method for isocratic HPLC analysis of polyamines
Abstract
A simple technique is described for the separation and analysis of polyamines in tissues and body fluids, utilizing precolumn clean-up on disposable CM-cellulose columns, followed by an automatable HPLC procedure. Complete separation and analysis takes 12-15 min per sample with sensitivity in the pmole range.