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Comparative Study
. 1977;15(3):239-45.
doi: 10.1016/0022-1759(77)90061-8.

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

M L Mahowald, B S Handwerger, E M Capertone Jr, S D Douglas
Comparative Study

A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation

M L Mahowald et al. J Immunol Methods. 1977.

Abstract

A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4 degrees C incubation period from 4 to 18 h enchanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lyphocytes by allowing accurate indentification of the central lymphocytes in morulas.

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