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. 1985 Apr;17(4):467-75.
doi: 10.1007/BF01003206.

A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry

G H Lewis, I D Bowen

A methacrylate embedding technique for combined autoradiography and acid phosphatase histochemistry

G H Lewis et al. Histochem J. 1985 Apr.

Abstract

A method is described that enables the simultaneous application of autoradiography and histochemistry in tissue sections prepared using a hydroxyethyl methacrylate (HEMA) embedding medium. A novel fixation regime, using a 1:9 v/v mixture of acetone and 10% neutral buffered formalin, improves section quality, histological staining and reduces tissue and cell shrinkage. The combined localization of [6-3H] thymidine incorporation and acid phosphatase in mouse thymus, duodenum, human stomach biopsy, earthworm and Walker tumour is described and counts of macrophages, phagocytosed cells, pyknotic cells, mitotic figures and thymidine-incorporating cells from young and old mouse thymus are presented.

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References

    1. Stain Technol. 1971 Mar;46(2):59-62 - PubMed
    1. J Microsc. 1982 May;126(Pt 2):183-9 - PubMed
    1. Stain Technol. 1979 Jan;54(1):5-12 - PubMed
    1. J Histochem Cytochem. 1980 Apr;28(4):308-10 - PubMed
    1. Histochemistry. 1976 Oct 7;49(1):43-50 - PubMed