Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2938:47-51.
doi: 10.1007/978-1-0716-4607-6_5.

Quantification of Lipid Droplets in Adipocytes

Affiliations

Quantification of Lipid Droplets in Adipocytes

Reabetswe R Pitere et al. Methods Mol Biol. 2025.

Abstract

Lipid droplets are cellular organelles that regulate various cellular process such as lipid and energy metabolism. The first morphological indication of adipogenesis is the accumulation of multiple small intracellular lipid droplets. Here, we describe a microscopy technique that can be used to quantify lipid droplets in adipocytes.

Keywords: ASCs; Adipocyte; Adipogenesis; Adipogenic differentiation; Lipid quantification.

PubMed Disclaimer

Similar articles

References

    1. Fader Kaiser CM, Romano PS, Vanrell MC, Pocognoni CA, Jacob J, Caruso B, Delgui LR (2022) Biogenesis and breakdown of lipid droplets in pathological conditions. Front Cell Dev Biol 9. https://doi.org/10.3389/fcell.2021.826248
    1. Zadoorian A, Du X, Yang H (2023) Lipid droplet biogenesis and functions in health and disease. Nat Rev Endocrinol 19:443–459. https://doi.org/10.1038/s41574-023-00845-0 - DOI - PubMed
    1. Chen L, Jin Y, Wu J, Ren Z (2022) Lipid droplets: a cellular organelle vital for thermogenesis. Int J Biol Sci 18:6176–6188. https://doi.org/10.7150/ijbs.77051 - DOI - PubMed - PMC
    1. Yang H, Liu J (2021) Chapter 11 – structure and function of lipid droplets. In: Ridgway ND, McLeod RS (eds) Biochemistry of lipids, lipoproteins and membranes, 7th edn. Elsevier, London, pp 357–394 - DOI
    1. Heid H, Rickelt S, Zimbelmann R, Winter S, Schumacher H, Dörflinger Y, Kuhn C, Franke WW (2014) On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9:e90386. https://doi.org/10.1371/journal.pone.0090386 - DOI - PubMed - PMC

LinkOut - more resources