Quantification of Lipid Droplets in Adipocytes
- PMID: 40445278
- DOI: 10.1007/978-1-0716-4607-6_5
Quantification of Lipid Droplets in Adipocytes
Abstract
Lipid droplets are cellular organelles that regulate various cellular process such as lipid and energy metabolism. The first morphological indication of adipogenesis is the accumulation of multiple small intracellular lipid droplets. Here, we describe a microscopy technique that can be used to quantify lipid droplets in adipocytes.
Keywords: ASCs; Adipocyte; Adipogenesis; Adipogenic differentiation; Lipid quantification.
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Similar articles
-
Automated lipid droplet quantification system for phenotypic analysis of adipocytes using CellProfiler.Toxicol Mech Methods. 2020 Jun;30(5):378-387. doi: 10.1080/15376516.2020.1747124. Epub 2020 Apr 8. Toxicol Mech Methods. 2020. PMID: 32208812 Free PMC article.
-
Depletion of Jmjd1c impairs adipogenesis in murine 3T3-L1 cells.Biochim Biophys Acta Mol Basis Dis. 2017 Jul;1863(7):1709-1717. doi: 10.1016/j.bbadis.2017.05.011. Epub 2017 May 10. Biochim Biophys Acta Mol Basis Dis. 2017. PMID: 28501567
-
Qualitative and quantitative analysis of lipid droplets in mature 3T3-L1 adipocytes using oil red O.STAR Protoc. 2024 Jun 21;5(2):102977. doi: 10.1016/j.xpro.2024.102977. Epub 2024 Jun 12. STAR Protoc. 2024. PMID: 38875117 Free PMC article.
-
Alpha-lipoic acid attenuates adipocyte differentiation and lipid accumulation in 3T3-L1 cells via AMPK-dependent autophagy.Life Sci. 2014 Apr 1;100(2):125-132. doi: 10.1016/j.lfs.2014.02.001. Epub 2014 Feb 14. Life Sci. 2014. PMID: 24530288
-
Automated Image Processing for Spatially Resolved Analysis of Lipid Droplets in Cultured 3T3-L1 Adipocytes.Tissue Eng Part C Methods. 2015 Jun;21(6):605-13. doi: 10.1089/ten.TEC.2014.0513. Epub 2014 Dec 18. Tissue Eng Part C Methods. 2015. PMID: 25390760 Free PMC article.
References
-
- Fader Kaiser CM, Romano PS, Vanrell MC, Pocognoni CA, Jacob J, Caruso B, Delgui LR (2022) Biogenesis and breakdown of lipid droplets in pathological conditions. Front Cell Dev Biol 9. https://doi.org/10.3389/fcell.2021.826248
-
- Zadoorian A, Du X, Yang H (2023) Lipid droplet biogenesis and functions in health and disease. Nat Rev Endocrinol 19:443–459. https://doi.org/10.1038/s41574-023-00845-0 - DOI - PubMed
-
- Chen L, Jin Y, Wu J, Ren Z (2022) Lipid droplets: a cellular organelle vital for thermogenesis. Int J Biol Sci 18:6176–6188. https://doi.org/10.7150/ijbs.77051 - DOI - PubMed - PMC
-
- Yang H, Liu J (2021) Chapter 11 – structure and function of lipid droplets. In: Ridgway ND, McLeod RS (eds) Biochemistry of lipids, lipoproteins and membranes, 7th edn. Elsevier, London, pp 357–394 - DOI
-
- Heid H, Rickelt S, Zimbelmann R, Winter S, Schumacher H, Dörflinger Y, Kuhn C, Franke WW (2014) On the formation of lipid droplets in human adipocytes: the organization of the perilipin-vimentin cortex. PLoS One 9:e90386. https://doi.org/10.1371/journal.pone.0090386 - DOI - PubMed - PMC
MeSH terms
LinkOut - more resources
Full Text Sources