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. 2025:2938:53-62.
doi: 10.1007/978-1-0716-4607-6_6.

RT-qPCR Quantification of Adipogenic Marker Genes (PPARg, C/EBPa, FABP4, and CD36)

Carina C Babbo  1 , Reabetswe R Pitere  1 , Rachel Giles  1 , Melvin A Ambele  1 , Michael S Pepper  1
Affiliations

RT-qPCR Quantification of Adipogenic Marker Genes (PPARg, C/EBPa, FABP4, and CD36)

Carina C Babbo et al. Methods Mol Biol. 2025.

Abstract

Adipogenesis is a complex multiphase process in which preadipocytes develop into adipocytes. This tightly regulated process is facilitated by a cascade of genes and transcription factors. In this chapter, we describe a protocol that can be used to quantify the expression of adipogenic markers using RT-qPCR.

Keywords: C/EBPa; CD36; FABP4; PPARg; RT-qPCR; adipogenesis.

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