Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Oct;164(1):466-9.
doi: 10.1128/jb.164.1.466-469.1985.

Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA

C J Smith

Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA

C J Smith. J Bacteriol. 1985 Oct.

Abstract

A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
    1. Appl Microbiol. 1974 May;27(5):961-79 - PubMed
    1. J Bacteriol. 1974 Sep;119(3):1072-4 - PubMed
    1. Am J Clin Nutr. 1974 Dec;27(12):1456-69 - PubMed
    1. Mol Gen Genet. 1979 Jan 5;168(1):111-5 - PubMed

MeSH terms

LinkOut - more resources