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. 2025 Apr;178(6):774-777.
doi: 10.1007/s10517-025-06415-w. Epub 2025 May 30.

Tumor Proteolytic Activity in Esophageal Cancer

E I Goufman  1 , N B Tikhonova  2 , K B Gershkovich  3 , A P Aleksankin  2 , A A Stepanov  2 , N V Nizyaeva  2 , V V Bochkov  2 , L M Mikhaleva  2 , O V Kovaleva  4 , I S Stilidi  4 , N E Kushlinskii  4
Affiliations

Tumor Proteolytic Activity in Esophageal Cancer

E I Goufman et al. Bull Exp Biol Med. 2025 Apr.

Abstract

The growth of malignant tumors is accompanied by an increase in plasmin activity, one of the key enzymes in oncogenesis. IgG proteolysis by plasmin yields fragments with exposed C-terminal lysine residues that can bind to plasmin heavy chain. Highly purified plasminogen (plasmin precursor) and heavy chains containing lysine-binding sites were isolated. The formation of IgG fragments as a result of their proteolysis by plasmin in solution was shown. Using ELISA with immobilized heavy chains, we found increased concentration of IgG fragments in the sera of patients with esophageal cancer in comparison with healthy donors. The test had high sensitivity (91%) and specificity (85%).

Keywords: IgG degradation products; enzyme-linked immunoassay; esophageal carcinoma; tumor proteolytic activity.

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References

    1. Liu CQ, Ma YL, Qin Q, et al. Epidemiology of esophageal cancer in 2020 and projections to 2030 and 2040. Thorac Cancer. 2023;14(1):3-11. https://doi.org/10.1111/1759-7714.14745 - DOI - PubMed
    1. Zhang Q, Zheng S, Liu Q, et al. Prognostic significance of pyruvate kinase M2 expression in esophageal squamous cell carcinoma and its meta-analysis. Transl Cancer Res. 2021;10(6):2643-52. https://doi.org/10.21037/tcr-21-442 - DOI - PubMed - PMC
    1. Yang Y, Huang X, Zhou L, et al. Clinical use of tumor biomarkers in prediction for prognosis and chemotherapeutic effect in esophageal squamous cell carcinoma. BMC Cancer. 2019;19(1):526. https://doi.org/10.1186/s12885-019-5755-5 - DOI - PubMed - PMC
    1. Kushlinskii NE, Gerstein ES. Tumor-associated proteases and their tissue inhibitors. Biological Markers of Tumors: Fundamental and Clinical Studies. Kushlinskii NE, Krasilnikov MA, eds. Moscow, 2017. P. 197-230. Russian.
    1. Pepper MS. Role of the matrix metalloproteinase and plasminogen activator-plasmin systems in angiogenesis. Arterioscler Thromb Vasc Biol. 2001;21(7):1104-17. https://doi.org/10.1161/hq0701.093685 - DOI - PubMed

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