Correction: Mechanism underlying the role of the circRNA OMA1/miR-654-3p/RAF1 axis in children with inflammatory bowel disease

Abstract

[This corrects the article DOI: 10.1007/s10616-025-00703-z.].

Fig. 5

Effects of circRNA OMA1-plasmid or miR-654-3p mimic on HT-29 cell viability, apoptosis, and inflammatory response. Control-plasmid, circRNA OMA1-plasmid, mimic control, or miR-654-3p mimic was transfected into HT-29 cells for 24 h. Subsequently, the cells were exposed to 2% DSS for 24 d. Cells were divided into six groups: control, DSS, DSS + control-plasmid, DSS + circRNA OMA1-plasmid, DSS + circRNA OMA1-plasmid + mimic control, or DSS + circRNA OMA1-plasmid + miR-654-3p mimic. A, B qRT-PCR analysis of circRNA OMA1 and miR-654-3p mRNA levels. C Cell viability was determined by MTT assay. D Flow cytometry analysis of cells apoptosis. E Quantification of apoptotic cells. F and G Western blot analysis of cleaved caspase-3. The secretion of TNF-α (H), IL-1β (I), and IL-6 (J) was determined by ELISA. **P < 0.01

Fig. 8

RAF1-siRNA reversed the effects of miR-654-3p inhibitor on cell viability, apoptosis and inflammatory response in HT-29 cells. Inhibitor control, miR-654-3p inhibitor, control-siRNA, or RAF1-siRNA were transfected into HT-29 cells for 24 h. Then, the cells were exposed to 2% DSS for 24 h. Cells were divided into six groups: control, DSS, DSS + controlplasmid, DSS + circRNA OMA1-plasmid, DSS + circRNA OMA1-plasmid + mimic control, or DSS + circRNA OMA1-plasmid + miR-654-3p mimic. A, B The mRNA levels of miR-654-3p and RAF1 were detected by RT-qPCR analysis. C and D Protein level of RAF1 was determined by western blot analysis. E MTT assay of cell viability. F Cell apoptosis was detected by flow cytometry assay. G Quantification of apoptotic cells. H and I Detection of cleaved caspase-3 expression using western blot assay. J The secretion of TNF-α, IL-1β, and IL-6 was assessed by ELISA. **P < 0.01