Identification of the major urinary metabolite of thromboxane B2 in the monkey
- PMID: 404671
- DOI: 10.1016/0090-6980(77)90234-9
Identification of the major urinary metabolite of thromboxane B2 in the monkey
Abstract
Thromboxane B2 (TxB2) was biosynthesized from prostaglandin endoperoxides (PGG2, PGH2) using guinea pig lung microsomes and infused into an unanesthetized monkey. Urine was collected and TxB2 metabolites were isolated by reversed phase partition chromatography and high performance liquid chromatography. A major metabolite (TxB2-M) was found to be excreted in greater than two-fold abundance relative to other metabolites. Its structure was determined by gas chromatography-mass spectrometry to be dinor-thromboxane B2. In vitro incubation of TxB2 with rat liver mitochondria yielded a C18 derivative with a mass spectrum identical to that of TxB2-M, substantiating that the major urinary metabolite of TxB2 in the monkey is a product of a single step of beta-oxidation.
Similar articles
-
Metabolism of thromboxane B2 in the monkey.J Biol Chem. 1978 Aug 10;253(15):5305-18. J Biol Chem. 1978. PMID: 97291
-
Metabolism of thromboxane B2 in man. Identification of the major urinary metabolite.J Biol Chem. 1977 Nov 10;252(21):7415-7. J Biol Chem. 1977. PMID: 914816
-
Metabolism of thromboxane B2 in the cynomolgus monkey.Prostaglandins. 1977 Apr;13(4):619-29. doi: 10.1016/0090-6980(77)90233-7. Prostaglandins. 1977. PMID: 404670
-
[Biosynthesis of prostaglandins; with special reference to prostaglandin endoperoxide and thromboxane].Seikagaku. 1976;48(12):1112-6. Seikagaku. 1976. PMID: 799720 Review. Japanese. No abstract available.
-
Beta-oxidation of eicosanoids.Prog Lipid Res. 1994;33(4):403-28. doi: 10.1016/0163-7827(94)90025-6. Prog Lipid Res. 1994. PMID: 7870740 Review. No abstract available.
Cited by
-
Prostaglandin-mediated hypercalcemia: a paraneoplastic syndrome.Klin Wochenschr. 1978 Apr 15;56(8):373-87. doi: 10.1007/BF01477292. Klin Wochenschr. 1978. PMID: 205705 Review.
-
Release of 15-keto-13, 14-dihydro-thromboxane B2 and prostaglandin D2 during anaphylaxis as measured by radioimmunoassay.Naunyn Schmiedebergs Arch Pharmacol. 1978 Dec;305(3):247-52. doi: 10.1007/BF00498818. Naunyn Schmiedebergs Arch Pharmacol. 1978. PMID: 740052 No abstract available.
-
Tromboxane B2 inhibits the pulmonary inactivation of prostaglandin E2 in the dog.Br J Pharmacol. 1980 Oct;70(2):295-9. doi: 10.1111/j.1476-5381.1980.tb07935.x. Br J Pharmacol. 1980. PMID: 7000216 Free PMC article.
-
The prostanoids in hemostasis and thrombosis: a review.Am J Pathol. 1980 Jun;99(3):743-804. Am J Pathol. 1980. PMID: 6992594 Free PMC article. Review. No abstract available.
-
Ionophores stimulate prostaglandin and thromboxane biosynthesis.Proc Natl Acad Sci U S A. 1977 Oct;74(10):4251-5. doi: 10.1073/pnas.74.10.4251. Proc Natl Acad Sci U S A. 1977. PMID: 270668 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources