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. 2025 May 31:18:2765-2777.
doi: 10.2147/IDR.S521802. eCollection 2025.

Antimicrobial Resistance and Genomic Characterization of Salmonella Serovars Typhimurium and 4,[5],12:i:- in Huzhou, China

Wei Yan  1 , Lei Ji  1 , Yunfeng Zha  1 , Fenfen Dong  1 , Deshun Xu  1
Affiliations

Antimicrobial Resistance and Genomic Characterization of Salmonella Serovars Typhimurium and 4,[5],12:i:- in Huzhou, China

Wei Yan et al. Infect Drug Resist. .

Abstract

Objective: Salmonella serovar Typhimurium (S. Typhimurium) and its monophasic variant, Salmonella 4,[5],12:i:-, have become two of the most frequently isolated serovars worldwide, in both humans and animals. This study investigated the antimicrobial resistance and genomic characteristics of these two serovar Salmonella.

Methods: Between 2021 and 2023, a total of 90 S. Typhimurium and Salmonella 4,[5],12:i:-were collected from clinical and food samples in Huzhou. Their antimicrobial resistance phenotype and genes, virulence genes, and phylogenetic relationship were analyzed.

Results: Salmonella 4,[5],12:i:-, which all belong to ST34, has become the main serotype of Salmonella isolated in Huzhou instead of S. Typhimurium. Notably, we observed a higher incidence of infections among the young population (<5 years old). The 90 Salmonella isolates were mainly resistant to tetracycline (94.4%), ampicillin (72.2%), and trimethoprim/sulfamethoxazole (70.0%), with multidrug resistance (MDR) rates as high as 93.3%. Genome sequencing indicated that these isolates possessed 39 antimicrobial resistance genes and 184 virulence genes.

Conclusion: This research enhances our understanding of S. Typhimurium and Salmonella 4,[5],12:i:- infections, which is helpful to guide clinical responses.

Keywords: Salmonella typhimurium; antimicrobial resistance; monophasic variant; multidrug resistance; whole genome sequencing.

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Conflict of interest statement

The authors declare no competing interests in this work.

Figures

Figure 1
Figure 1
Epidemiological information regarding the 90 isolates in this study: (a) number of isolates in each age group; (b) composition ratios of serotypes in each age group. (c) composition ratios of ST types in each age group; and (d) composition ratios of sex in each age group.
Figure 2
Figure 2
Annotation heatmap of AMR genes among 90 isolates.
Figure 3
Figure 3
Annotation heatmap of virulence factors among 90 isolates.
Figure 4
Figure 4
Core genome SNP-based phylogenomic tree of all 90 Salmonella isolates.
See this image and copyright information in PMC

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References

    1. Issenhuth-Jeanjean S, Roggentin P, Mikoleit M, et al. Supplement 2008-2010 (no. 48) to the White-Kauffmann-Le Minor scheme. Res Microbiol. 2014;165(7):526–530. doi:10.1016/j.resmic.2014.07.004 - DOI - PubMed
    1. Buckle GC, Walker CL, Black RE. Typhoid fever and paratyphoid fever: systematic review to estimate global morbidity and mortality for 2010. J Glob Health. 2012;2(1):010401. doi:10.7189/jogh.02.010401 - DOI - PMC - PubMed
    1. Hohmann EL, Hohmann EL. Nontyphoidal salmonellosis. Clin Infect Dis. 2001;32(2):263–269. doi:10.1086/318457 - DOI - PubMed
    1. Ohl ME, Miller SI. Salmonella: a model for bacterial pathogenesis. Annu Rev Med. 2001;52(1):259–274. doi:10.1146/annurev.med.52.1.259 - DOI - PubMed
    1. Xu Y, Zhou X, Jiang Z, et al. Epidemiological Investigation and Antimicrobial Resistance Profiles of Salmonella Isolated From Breeder Chicken Hatcheries in Henan, China. Front Cell Infect Microbiol. 2020;10:497. doi:10.3389/fcimb.2020.00497 - DOI - PMC - PubMed

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