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. 2025 Jun 5.
doi: 10.1007/s10528-025-11150-y. Online ahead of print.

POLR2H Serves as a Novel Prognostic Biomarker Correlated with Immune Infiltration in Prostate Cancer

Aiyun Han  1 , Shen Li  2 , Jin Zhang  2 , Wenping Li  3
Affiliations

POLR2H Serves as a Novel Prognostic Biomarker Correlated with Immune Infiltration in Prostate Cancer

Aiyun Han et al. Biochem Genet. .

Abstract

Prostate cancer is a prevalent malignancy, and effective biomarkers are needed to improve patient management. This study aimed to explore the prognostic value and potential functions of POLR2H in prostate cancer. Prognosis-related genes for prostate cancer were screened through high-throughput functional screening using CRISPR. qPCR was used to detect POLR2H expression in different prostate cancer cell lines, prostate hyperplasia cell lines, and normal human prostate epithelial cells. Transwell and scratch assays were conducted to assess cell migration ability. Cell proliferation ability was evaluated by CCK-8 and colony formation assays. POLR2H emerged as a gene of interest associated with patient outcomes. Its expression was found to be elevated in prostate cancer cell lines compared with benign and normal prostate cells. Functional assays indicated that altering POLR2H levels affected cell migration and proliferation. Moreover, analysis across patient cohorts suggested an association between POLR2H expression and immune cell infiltration, implying a potential role in immunotherapy response. These findings support the role of POLR2H as a promising prognostic marker and potential therapeutic target in prostate cancer.

Keywords: Biomarker; POLR2H; Prostate cancer.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare no competing interests. Ethical Approval: The study protocol was performed in accordance with the Declaration of Helsinki and approved by the human ethics committee of Shijiazhuang Hospital (Shijiazhuang, China) (identifier: 2024/006). All patients signed informed consent forms and publish declarations before samples being collected.

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