Exploring the immunomodulatory mechanism of total glucosides of paeony on Sjögren's syndrome dry eye disease based on the "gut-eye axis" pathway
- PMID: 40471444
- DOI: 10.1007/s10792-025-03607-1
Exploring the immunomodulatory mechanism of total glucosides of paeony on Sjögren's syndrome dry eye disease based on the "gut-eye axis" pathway
Abstract
Purpose: Sjögren's syndrome-related dry eye disease (SS-DED) is an autoimmune disorder characterized by ocular surface inflammation and lacrimal gland dysfunction, with limited clinical treatment options currently available.
Methods: Based on the traditional Chinese medicine theory of "the liver opens into the eyes" and the modern "gut-eye axis" hypothesis. This study investigated the therapeutic effects and immunomodulatory mechanisms of total glucosides of paeony (TGP) using a NOD mouse model.
Results: The results showed that TGP significantly increased tear secretion in SS mice, alleviated corneal and conjunctival damage, improved pathological changes in the Harderian gland, reduced inflammatory infiltration, and restored glandular secretory function. At the molecular level, TGP markedly downregulated pro-inflammatory cytokines such as IL-6 and IL-17 while upregulating anti-inflammatory factors like IL-10 and TGF-β, effectively modulating the Th17/Treg immune balance. Additionally, TGP enhanced the expression of tight junction proteins (ZO-1, Occludin, and Claudin-3) in SS mice, repairing intestinal barrier function. 16S rRNA sequencing analysis revealed that TGP intervention regulated gut microbiota composition in SS mice, reducing the Firmicutes/Bacteroidetes ratio and increasing the abundance of beneficial bacteria such as Lactobacillus. Functional prediction analysis indicated that TGP-modulated gut microbiota primarily participated in key metabolic pathways, including carbohydrate and amino acid metabolism.
Conclusion: In conclusion, TGP can ameliorate ocular surface damage in SS mice by regulating gut microecology and immune homeostasis via the "gut-eye axis" pathway. Our study not only provides a novel therapeutic strategy for SS-DED but also offers scientific evidence for the modernization of traditional Chinese medicine theories.
Keywords: Gut microbiota; Gut-eye axis; Immunomodulation; Sjögren’s syndrome-related dry eye; Total glucosides of paeony.
© 2025. The Author(s), under exclusive licence to Springer Nature B.V.
Conflict of interest statement
Declarations. Conflict of interest: The authors declared that they have no conflicts of interest regarding this work. Ethics approval and consent to participate: The animal experiments strictly adhered to national guidelines on laboratory animal welfare and ethics and were approved by the Institutional Animal Ethics Committee (Approval No.: SYYXY2024091101). Consent for publication: Not Applicable.
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