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. 2025 Oct;113(2):116934.
doi: 10.1016/j.diagmicrobio.2025.116934. Epub 2025 May 30.

A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance

Madeline E Spradley  1 , Timothy Williams  2 , Rendie McHenry  3 , Laura L Short  3 , Dalton J Nelson  4 , James D Chappell  3 , Natasha Halasa  5 , Frederick R Haselton  4 , Jonathan E Schmitz  6
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Free article

A pooled testing strategy for enterovirus D68 to facilitate local, resource-conserving surveillance

Madeline E Spradley et al. Diagn Microbiol Infect Dis. 2025 Oct.
Free article

Abstract

Pooled testing represents a resource-conserving diagnostic strategy, and we propose enterovirus-D68 (EV-D68) as an attractive target for pooling (especially locally). To validate an RT-qPCR-based approach, retrospective pools were contrived from EV-D68-positive nasopharyngeal specimens and non-EV-D68-enterovirus-positive specimens. Five/nine-fold pools demonstrated 90.9 %/88.6 % sensitivity versus-unpooled testing (no false-positives), with commensurate results observed prospectively.

Keywords: Enterovirus D68; Outbreak surveillance; Pooled testing; RT-qPCR; Respiratory virus.

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Conflict of interest statement

Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jonathan Schmitz reports financial support was provided by Vanderbilt University Medical Center. Rick Haselton reports financial support was provided by Vanderbilt University. James Chappell reports a relationship with Vanderbilt University Medical Center that includes: funding grants. Natasha Halasa reports a relationship with Vanderbilt University Medical Center that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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