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. 2025 Jun 5.
doi: 10.1007/s00266-025-04970-1. Online ahead of print.

Impact of Poly-L-Lactic Acid Reconstitution on the Neocollagenesis Profile

Janaína Motta Guerra  1 , Maria Carolina Portela Albuquerque  1 , Clarice Lioba de Araújo  1 , Júlia Gonçalves Montenegro  1 , Diana Araújo Cunha  1 , Fabrício Bitu Sousa  1 , Paulo Goberlânio de Barros Filho  1 , Átila Vinícius Vitor Nobre  2
Affiliations

Impact of Poly-L-Lactic Acid Reconstitution on the Neocollagenesis Profile

Janaína Motta Guerra et al. Aesthetic Plast Surg. .

Abstract

Purpose: The objective of the present work was to evaluate the percentage of neocollagenesis induction of the Sculptra® in different forms of reconstitution.

Methods: For this, 150 male Swiss mice were used to which saline solution or reconstituted Sculptra® were applied to the back subcutaneous tissue immediately before application (0 h), 2, 24 or 72 h before administration and were euthanized after 15, 30 and 60 days of administration of the material in a number of 10/group/day. Their backs were removed for histological analysis and immunoexpression for alpha-smooth muscle actin (α-SMA). The data was statistically analyzed (p < 0.05).

Results: After 30 days of administration, the Sculptra® 72 h group showed greater collagen formation than the control (p = 0.016) and 0h groups (p = 0.023). After 60 days, the Sculptra® 24 and 72 h groups also showed greater collagen formation than the control (p = 0.025) and 0 h groups ( p = 0.039). After 30 days of administration, the Sculptra® 24 and 72 h groups showed higher immunoexpression for α-SMA (p = 0.001), as well as after 60 days (p < 0.001).

Conclusions: We can conclude that Sculptra® reconstituted 2, 24 and 72 h before its application has the potential to induce collagen after 60 days. Furthermore, there is greater expression of α-SMA after reconstitution 24 and 72 h before Sculptra® injection, after 30 and 60 days. No level of evidence is needed for Basic Science, Animal Study, Cadaver Study, and Experimental Study Articles.

No level assigned: This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Keywords: Collagen; Dermal fillers; Poly-L-lactic acid.

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Conflict of interest statement

Declarations. Conflict of Interest: The authors declare that they have no conflicts of interest to disclose. Ethical Approval: This work was submitted to the Ethics Committee for the Use of Animals (protocol 023/21). Informed Consent: For this type of study, informed consent is not required.

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