Scurvy; a comparison between ultrastructural and biochemical changes observed in cultured fibroblasts and the collagen they synthesise
- PMID: 404754
- DOI: 10.1007/BF02889141
Scurvy; a comparison between ultrastructural and biochemical changes observed in cultured fibroblasts and the collagen they synthesise
Abstract
Earlier biochemical investigations of cultured 3T6 fibroblasts have shown that ascorbate deficiency has no effect on the synthesis of collagen protein quantitatively but does produce inhibition of the critical post-translational hydroxylation of collagen essential for normal fibrogenesis and of formation of hydroxylysine-derived cross-links. This ultrastructural study on the same 3T6 fibroblast system demonstrates that ascorbate deficiency does not affect the cell morphology, particularly that of the protein synthetic or secretory apparatus, but does prevent the deposition of typical 640A degrees banded collagen fibres; instead, finer, unbanded fibrils--presumably collagenous--are deposited extracellularly. This confirms the earlier biochemical findings as wll as presenting a new finding--the inability of ascorbate-deficient cells to lay down normal collagen fibrils; possible mechanisms are considered in terms of the known biochemical lesions. The tissue culture findings are also compared with those observed in vivo in the scorbutic guinea-pig, where other workers have reported biochemical and ultrastructural evidence that collagen synthesis is inhibited. The apparently paradoxical observations in the two systems are considered; we conclude that the tissue culture system demonstrates the primary collagenous lesion which is perhaps obscured in vivo by secondary effects--nevertheless the two approaches are complementary.
Similar articles
-
Influence of ascorbic acid on ribosomal patterns and collagen biosynthesis in healing wounds of scorbutic guinea pigs.Biochem J. 1974 Sep;142(3):641-51. doi: 10.1042/bj1420641. Biochem J. 1974. PMID: 4464846 Free PMC article.
-
Studies in vivo on the biosynthesis of collagen and elastin in ascorbic acid-deficient guinea pigs.Biochem J. 1969 Jun;113(2):387-97. doi: 10.1042/bj1130387. Biochem J. 1969. PMID: 4309121 Free PMC article.
-
Coordinate regulation of collagen and proteoglycan synthesis in costal cartilage of scorbutic and acutely fasted, vitamin C-supplemented guinea pigs.Arch Biochem Biophys. 1986 Apr;246(1):42-51. doi: 10.1016/0003-9861(86)90447-9. Arch Biochem Biophys. 1986. PMID: 3963829
-
Ascorbate requirement for hydroxylation and secretion of procollagen: relationship to inhibition of collagen synthesis in scurvy.Am J Clin Nutr. 1991 Dec;54(6 Suppl):1135S-1140S. doi: 10.1093/ajcn/54.6.1135s. Am J Clin Nutr. 1991. PMID: 1720597 Review.
-
Vitamin C and bone markers: investigations in a Gambian population.Proc Nutr Soc. 2003 May;62(2):429-36. doi: 10.1079/pns2003247. Proc Nutr Soc. 2003. PMID: 14506891 Review.
Cited by
-
Small changes huge impact: the role of protein posttranslational modifications in cellular homeostasis and disease.J Amino Acids. 2011;2011:207691. doi: 10.4061/2011/207691. Epub 2011 Jul 21. J Amino Acids. 2011. PMID: 22312457 Free PMC article.
-
Collagen metabolism: a comparison of diseases of collagen and diseases affecting collagen.Am J Pathol. 1980 Jan;98(1):225-80. Am J Pathol. 1980. PMID: 6985771 Free PMC article. Review. No abstract available.
-
Procollagen mRNA metabolism during the fibroblast cell cycle and its synthesis in transformed cells.Nucleic Acids Res. 1980 Jun 25;8(12):2823-33. doi: 10.1093/nar/8.12.2823. Nucleic Acids Res. 1980. PMID: 6253892 Free PMC article.
-
Metastatic emphysema: a mechanism for acquiring inguinal herniation.Ann Surg. 1981 Sep;194(3):270-8. doi: 10.1097/00000658-198109000-00004. Ann Surg. 1981. PMID: 7023395 Free PMC article.
-
Connective tissue: an eclectic historical review with particular reference to the liver.Histochem J. 1981 May;13(3):341-96. doi: 10.1007/BF01005055. Histochem J. 1981. PMID: 7019165 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources