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. 2025 May 21:2025:10.17912/micropub.biology.001492.
doi: 10.17912/micropub.biology.001492. eCollection 2025.

Genome Sequences of Microbacterium foliorum Phages BouleyBill and Carostasia, isolated in France

Christophe Regeard  1 , Florence Constantinesco-Becker  1 , Anne Lopes  1 , Ana A Arteni  1 , Malika Ouldali  1 , Laura Pieri  1 , Monique Auberdiac  2 , Daniel Delaruelle  2 , Kevin Tambosco  2 , Hakima Abes  2 , Clément Almeida-Monge  3 , Félix Benard  3 , Lucie Boucard  3 , Elsa Chaouat  3 , Juliette Charazac  3 , Caroline Comte  3 , Marie Coutard  3 , Téo Denis  3 , Clarisse Deschamps-Martin  3 , Erwin Filloux  3 , Anastasia Gaultier  3 , Madeleine Gautheret  3 , Hafsa Harrat  3 , Océane Hill  3 , Mattéo Jalmain  3 , Cécile Jolivet  3 , Diane Le Tyrant  3 , Sarah Lopez  3 , Cléa Medin  3 , Camille Outtier  3 , Mélissa Roze  3 , Maria Rubio-Espinal  3 , May-Blue Zeni  3 , Ombeline Rossier  1
Affiliations

Genome Sequences of Microbacterium foliorum Phages BouleyBill and Carostasia, isolated in France

Christophe Regeard et al. MicroPubl Biol. .

Abstract

Bacteriophages BouleyBill and Carostasia, exhibiting siphovirus morphology, were isolated in France. Both infected Microbacterium foliorum strain NRRL B-24224. Their 39,215-bp and 40,393-bp genomes were assigned to subclusters EA4 and EA10, respectively, widening for the first time the known geographical distribution of these subclusters to Europe.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. <b>Plaque and particle morphologies for BouleyBill (A and B) and Carostasia (C and D)</b>
Figure 1. Plaque and particle morphologies for BouleyBill (A and B) and Carostasia (C and D)
(A and C) Clear plaques observed in the LB agar overlay supplemented with M . foliorum strain NRRL B-24224 and 1 mM CaCl2 after 24 h at 30°C. (B and D) Negative staining of phages with 2% wt/vol uranyl acetate observed with a Tecnai Spirit microscope operated at 100 kV (TFS) and equipped with a K2 Base 4kx4k direct electron detection camera (Ametek/Gatan). The magnification used was 19,800× with a pixel size of 2.5 Å at the level of the specimen. Scale bar: 100 nm.
See this image and copyright information in PMC

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