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. 2025 Jun 3;7(6):000918.v4.
doi: 10.1099/acmi.0.000918.v4. eCollection 2025.

Culture harder: use more specimens to increase methicillin-resistant Staphylococcus aureus culture yield relative to PCR

Arvette E Mitchell  1 , Arpit P Patel  1 , Jennifer DiCandilo  1 , Zachary W Rebollido  2 , Matthew A Pettengill  1
Affiliations

Culture harder: use more specimens to increase methicillin-resistant Staphylococcus aureus culture yield relative to PCR

Arvette E Mitchell et al. Access Microbiol. .

Erratum in

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) causes considerable morbidity and mortality in both community-acquired and healthcare-associated infections, but detecting colonization with MRSA has been shown to improve patient outcomes in certain clinical settings. MRSA colonization detection has been carried out in a variety of ways, with molecular assays having superior sensitivity in most studies relative to culture, but culture is disadvantaged in some comparisons by utilization of low specimen volumes. We compared a commercial molecular assay to both low-volume (10 µl) and high-volume (650 µl) cultures and found that increasing the volume utilized for culture led to the detection of 25% more cases than low-volume culture.

Keywords: PCR; culture; methicillin-resistant Staphylococcus aureus (MRSA) screening.

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Conflict of interest statement

M.P. has received research funds from Cepheid, Inc., and no other authors have any relevant conflicts of interest to declare.

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References

    1. Hanberger H, Walther S, Leone M, Barie PS, Rello J, et al. Increased mortality associated with methicillin-resistant Staphylococcus aureus (MRSA) infection in the intensive care unit: results from the EPIC II study. Int J Antimicrob Agents. 2011;38:331–335. doi: 10.1016/j.ijantimicag.2011.05.013. - DOI - PubMed
    1. Delaney JA, Schneider-Lindner V, Brassard P, Suissa S. Mortality after infection with methicillin-resistant Staphylococcus aureus (MRSA) diagnosed in the community. BMC Med. 2008;6:2. doi: 10.1186/1741-7015-6-2. - DOI - PMC - PubMed
    1. Meng L, Pourali S, Hitchcock MM, Ha DR, Mui E, et al. Discontinuation patterns and cost avoidance of a pharmacist-driven methicillin-resistant Staphylococcus aureus nasal polymerase chain reaction testing protocol for de-escalation of empiric vancomycin for suspected pneumonia. Open Forum Infect Dis. 2021;8:ofab099. doi: 10.1093/ofid/ofab099. - DOI - PMC - PubMed
    1. Dalpke AH, Hofko M, Stock C, Zimmermann S. Evaluation of the BD Max MRSA XT assay for use with different swab types. J Clin Microbiol. 2014;52:4343–4346. doi: 10.1128/JCM.02306-14. - DOI - PMC - PubMed
    1. Yarbrough ML, Warren DK, Allen K, Burkholder D, Daum R, et al. Multicenter evaluation of the Xpert MRSA NxG assay for detection of methicillin-resistant Staphylococcus aureus in nasal swabs. J Clin Microbiol. 2018;56:e01381-17. doi: 10.1128/JCM.01381-17. - DOI - PMC - PubMed

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