Nasibacterium caprae gen. nov., sp. nov., isolated from a goat with respiratory disease

Abstract

A Gram-stain-negative, aerobic and rod-shaped bacteria, designated ZY210820^T, was isolated from the nasal cavity of a goat with respiratory disease in Yunnan Province, PR China. The strain grew at pH 6.0-9.0 (optimum pH 7.0), at 24-39 °C (optimum 37 °C) and with 0.5-2.0% (w/v) NaCl (optimum 1.0% NaCl). Phylogenetic analysis of 16S rRNA gene sequences showed that the strain formed a separated branch within the family Moraxellaceae with the highest similarity of 93.1% to Acinetobacter kanungonis JCM 34131^T. Phylogenomic analysis of 327 single-copy protein sequences revealed that the strain belonged to the family Moraxellaceae and constituted a separated branch. The genomic G + C content was 35.5%. The highest orthologous average nucleotide identity (OrthoANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between the strain and the type strains in the family Moraxellaceae were 72.5% (Acinetobacter calcoaceticus DSM 30006^T), 37.0% (Fluviicoccus keumensis JCM 19370^T), and 64.0% (Acinetobacter kanungonis JCM 34131^T), respectively. The predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, cardiolipin, monolysocardiolipin, and diacylglycerol. The strain contained C_12:0, C_16:0, summed feature 3 (C_16:1ω7c and/ or C_16:1ω6c), C_17:0, and C_15:1ω6c as the major fatty acid (> 5%) and CoQ-9 as the major respiratory quinone. The results of the polyphasic analysis revealed that strain ZY210820^T represents a novel species of a new genus in the family Moraxellaceae, and the name Nasibacterium caprae gen. nov., sp. nov. is proposed. The type strain is ZY210820^T (= CCTCC AB 2021475^T = NBRC 115474^T).

Keywords: Nasibacterium caprae; Goat; Respiratory disease; Taxonomic position; Virulence gene.