Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Sep 11;13(17):6075-88.
doi: 10.1093/nar/13.17.6075.

Isolation of polymorphic DNA segments from human chromosome 21

P C Watkins, R E Tanzi, K T Gibbons, J V Tricoli, G Landes, R Eddy, T B Shows, J F Gusella
Free PMC article

Isolation of polymorphic DNA segments from human chromosome 21

P C Watkins et al. Nucleic Acids Res. .
Free PMC article

Abstract

A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction fragment length polymorphisms to provide genetic markers on chromosome 21. Nine independently isolated clones used as probes identified a total of 11 new RFLPs. Four of the DNA probes recovered from the library have been mapped unequivocally to chromosome 21 using a panel of somatic cell hybrid lines. A fifth probe detected an RFLP on chromosome 21 as well as sequences on other chromosomes. This set of RFLPs may now form the basis for construction of a genetic linkage map of human chromosome 21.

PubMed Disclaimer

References

    1. Exp Cell Res. 1977 Mar 1;105(1):109-17 - PubMed
    1. In Vitro. 1984 Nov;20(11):856-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3259-63 - PubMed
    1. Cytogenet Cell Genet. 1978;21(1-2):99-104 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3382-6 - PubMed

Publication types