[Histological and immunohistochemical investigations on the transplanted isolated islets of Langerhans in diabetic rats (author's transl)]

Abstract

Isologous isolated islets of LANGERHANS were transplanted in the peritoneum and through the portal vein in the liver of diabetic rats. With the help of histological and immunohistochemical investigations, estimation of blood sugar and glucose tolerance tests was tested over a period of 12 month, whether the peritoneum or the liver is more suited as transplantation site for isolated islets of LANGERHANS. After transplantation of islets of LANGERHANS in the peritoneum of diabetic rats the blood glucose decreased and this effect could be maintained over a period of three months. The histological investigations showed strong periinsulinar reactions and the immunohistochemical insulin and glucagon proofs prospered over a period of three months. After transplantation of isolated islets in the liver it resulted in a normalization of blood glucose levels up to 12 months after transplantation and in this time prospered the immunohistochemical insulin and glucagon proof. These results have suggested, that the liver is more suited as transplantation site for isolated islets of LANGERHANS than the peritoneum.