In-ovo stimulation trains innate immunity to mitigate Campylobacter jejuni in broiler chickens

Abstract

Campylobacter is the most reported zoonotic pathogen in Europe and controlling it in broiler chickens has gained much attention among the scientists especially, considering its commensalism in chicken gut. In a previous study, we demonstrated the potential of reducing Campylobacter jejuni colonization in the ceca of broiler chickens (35 days old) by in-ovo stimulation with the probiotic Leuconostoc mesenteroides B/00288 strain alone (probiotic) and in combination with garlic aqueous extract (prophybiotic). The current study further investigated the physiological and genomic responses of these birds to elucidate the potential mechanisms behind the reduction of Campylobacter observed in the ceca. The quantification of the relative abundances of selected bacterial communities in the ceca and the expression of immune-related genes in the cecal mucosa, cecal tonsils, spleen and liver was performed using qPCR. Histomorphology of the ceca was analysed by PAS staining method. The serum immunoglobulin Y (IgY) content was quantified using an ELISA method. Both treatments reduced the abundance of Akkermansia sp. and the probiotic treatment reduced Bifidobacterium sp. in the ceca of Campylobacter infected chickens when compared to the control birds (in-ovo injected with physiological saline). A higher innate immune defence response was observed through gene expression in the cecal tonsils and spleen of the in-ovo stimulated birds when compared to the control birds. The villus width and crypt depth were decreased in the in-ovo stimulated groups when compared to the control group while the serum IgY content did not differ between the groups. In conclusion, this study provides evidence that in-ovo stimulation can be an effective method in innate immunity training to control Campylobacter jejuni in broiler chickens.

Keywords: Broiler; Campylobacter jejuni; In-ovo stimulation; Innate immunity.

Fig. 1

. Production parameters of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: Slaughter weight B: Post-infection feed conversion ratio.

Fig. 2

Relative abundance changes in cecal bacteria of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: Bifidobacterium sp. B: Akkermansia sp.

Fig. 3

Cecal histomorphology changes of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: Villus width B: Crypt depth.

Fig. 4

Serum immunoglobulin Y content of in-ovo treated broiler chickens infected with Campylobacter jejuni.

Fig. 5

CLDN1 expression in the cecal mucosa of in-ovo treated broiler chickens infected with Campylobacter jejuni.

Fig. 6

Gene expression in the cecal tonsils of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: IL1B B: IL2 C: IL8 D: IL10 E: FFAR2 F: AVBD1.

Fig. 7

Gene expression in the spleen of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: IL6 B: IL8 C: IL10 D: AVBD1 E: CLDN.

Fig. 8

Gene expression in the liver of in-ovo treated broiler chickens infected with Campylobacter jejuni. A: IL2 B: IL6 C: IL10 D: CATHL2 E: AVBD1.