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. 2025 Jun 9;82(7):330.
doi: 10.1007/s00284-025-04298-8.

Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis

Le Duy Khuong  1 , Van Minh Nguyen  2 , Roan Thi Do  3 , Huong Thi Thanh Doan  3 , Don Nguyen  4 , Sang Van Vu  5 , Hong Thi Nguyen  6   7 , Ichiro Kamei  8 , Chu Luong Tri  9
Affiliations

Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis

Le Duy Khuong et al. Curr Microbiol. .

Abstract

The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as Bacillus safensis, a newly discovered microorganism capable of synthesizing FPUase. FPUase production conditions were optimized through single‑factor experiments analyzing fermentation time, substrate concentration, pH, and temperature. Using the Box-Behnken Design (BBD) under the response surface methodology (RSM), the study refined these factors to enhance FPUase production. The resulting second-degree polynomial model demonstrated high validity (R2 = 0.9913), and statistically significant interactions (P < 0.05). Optimal conditions-12 g/L of substrate concentration, pH 7, and a fermentation temperature of 40 °C-yielded the highest FPUase activity of 61.5 U/L.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that they have no conflict of interest. Ethical Approval: Not applicable. Consent to Participate: Not applicable. Consent for Publication: Not applicable.

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