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. 2025 May 22;34(11):2643-2655.
doi: 10.1007/s10068-025-01902-2. eCollection 2025 Jul.

Anti-inflammatory effects of naringinase treated ethanol extracts of Poncirus trifoliata fruit

Kyung Eun Moon  1 , Hyeon Hwa Oh  1 , Byung-Min Oh  1 , Young-Soo Kim  1
Affiliations

Anti-inflammatory effects of naringinase treated ethanol extracts of Poncirus trifoliata fruit

Kyung Eun Moon et al. Food Sci Biotechnol. .

Abstract

In this study, the flavonoids in Poncirus trifoliata fruit ethanol extract (PFE) were identified using UPLC-Q-TOF-MS and their anti-inflammatory effects on Raw 264.7 cells were evaluated. The 40% ethanol extract showed highest concentrations of total phenolic content (TPC), total flavonoid content (TFC), and flavonoids. In naringinase-treated PFE powder (NPFEP), poncirin decreased from 76.47 to 68.62 mg/g (13.20 mM), and isosakuranetin increased to 3.53 mg/g (12.33 mM). NPFEP demonstrated superior anti-inflammatory properties in Raw 264.7 cells compared to naringinase-untreated PFEP (UNPFEP), with decreased ROS production, lower inflammatory mediators (NO and PGE2), extracellular cytokines (TNF-α and IL-1β), and downregulation of COX-2 and intracellular cytokines (IL-1β and IL-6) gene expression. UPLC analysis of NPFEP identified four flavanones (poncirin, naringin, isosakuranetin, and naringenin), one chalcone (2',6'-dihydroxy-4-methoxychalcone-4'-O-neohesperid), and two triterpenes (cucurbitacin D and esculentic acid), which likely contribute to the observed anti-inflammatory effect.

Keywords: Anti-inflammatory; Flavonoid; Naringinase; Poncirin; Poncirus trifoliate.

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Conflict of interest statement

Conflict of interestThe authors declare no conflicts of interest.

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References

    1. An SJ, Pae HO, Oh GS, Choi BM, Jeong S, Jang SI, Oh H, Kwon TO, Song CE, Chung HT. Inhibition of TNF-α, IL-1β, and IL-6 productions and NF-κB activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated from Catalpa ovata G. Don (Bignoniaceae). International Journal of Immunopharmacology. 2:1173-1181 (2022) - PubMed
    1. Benka M, Görlitz K, Schöttgen MC, Lagies S, Mohl DA, Kather M, Kammerer B. Mass spectrometric analysis of cucurbitacins and dihydrocucurbitacins from the tuber of Citrullus naudinianus. Biomolecules. 13:1168-1182 (2023) - PMC - PubMed
    1. Bodakowska-Boczniwiecz J, Garncarek Z. Immobilization of Naringinase from Penicillium decumbens on Chitosan Microspheres for Debittering Grapefruit Juice. Molecules. 24:4234-4251 (2019) - PMC - PubMed
    1. Cao X, Guo X, Fang X, Ru S, Li E. Effects of poncirin, a citrus flavonoid and its aglycone, isosakuranetin, on the gut microbial diversity and metabolomics in mice. Molecules. 27:3641-3654 (2022) - PMC - PubMed
    1. Cui L, Wang X, Lu J, Tian J, Wang L, Qu J, Wei J. Rapid identification of chemical constituents in Artemisia argyi L´evi. et Vant by UPLC-Q-exactive-MS/ MS. Journal of Food Quality. 5597327:1-7 (2021)

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