Industrial-scale antifungal film incorporated with Cinnamomum cassia oil: enhancing bread quality attributes and evaluation of organoleptic properties

Yuncheong Choi^#^{1

2}, Ji Sou Lyu^#^{1

3}, Jung-Soo Lee⁴, Yoonjee Chang⁵, Jaejoon Han^{1

6}

Affiliations

¹ Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 02841 Republic of Korea.
² Team of Packaging Solution, Department of Fundamental Technology, Lotte R&D Center, 201, Magokjungang-ro, Gangseo-gu, Seoul, 07594 Republic of Korea.
³ Institute of Control Agents for Microorganisms, Korea University, Seoul, 02841 Republic of Korea.
⁴ Biopolymers and Colloids Laboratory, Department of Food Science, University of Massachusetts, Amherst, MA 01003 USA.
⁵ Department of Food and Nutrition, College of Science and Technology, Kookmin University, Seoul, 02707 Republic of Korea.
⁶ Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841 Republic of Korea.

^# Contributed equally.

PMID: 40492040
PMCID: PMC12145359 (available on 2026-04-28)
DOI: 10.1007/s10068-025-01883-2

Industrial-scale antifungal film incorporated with Cinnamomum cassia oil: enhancing bread quality attributes and evaluation of organoleptic properties

Yuncheong Choi et al. Food Sci Biotechnol. 2025.

. 2025 Apr 28;34(11):2519-2528.

doi: 10.1007/s10068-025-01883-2. eCollection 2025 Jul.

Authors

Yuncheong Choi^#^{1

2}, Ji Sou Lyu^#^{1

3}, Jung-Soo Lee⁴, Yoonjee Chang⁵, Jaejoon Han^{1

6}

Affiliations

¹ Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul, 02841 Republic of Korea.
² Team of Packaging Solution, Department of Fundamental Technology, Lotte R&D Center, 201, Magokjungang-ro, Gangseo-gu, Seoul, 07594 Republic of Korea.
³ Institute of Control Agents for Microorganisms, Korea University, Seoul, 02841 Republic of Korea.
⁴ Biopolymers and Colloids Laboratory, Department of Food Science, University of Massachusetts, Amherst, MA 01003 USA.
⁵ Department of Food and Nutrition, College of Science and Technology, Kookmin University, Seoul, 02707 Republic of Korea.
⁶ Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841 Republic of Korea.

^# Contributed equally.

PMID: 40492040
PMCID: PMC12145359 (available on 2026-04-28)
DOI: 10.1007/s10068-025-01883-2

Abstract

This research aims to develop an antifungal packaging film to mitigate the quality deterioration of mass-produced bread for industrial purposes. Various antifungal agents were tested against four target fungi; Aspergillus niger, A. flavus, Penicillium corylophilum, and Neurospora sp.; commonly found in bread, to replace the commercial antifungal film. Among them, Cinnamomum cassia essential oil (CEO) exhibited strong antifungal activity, with MIC values below 100 ppm against all tested strains. We implemented the CEO-incorporated powder onto the multilayered packaging film and subsequent packaging trials were conducted on the industrial packaging machinery. Organoleptic analyses using electronic tongue and electronic nose revealed that the application of CEO maintained the bread's flavor and aroma throughout storage, implying the newly developed CEO-treated film was comparably preferred to the conventional material. Therefore, this investigation highlights the promising potential of CEO's effective antifungal agent to combat bread quality deterioration during distribution for industrial operations.

Keywords: Active food packaging,; Cinnamomum cassia bark essential oil; Electronic sensory evaluation; Industrial scale; Natural antifungal agent.

© The Korean Society of Food Science and Technology 2025. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Conflict of interest statement

Conflict of interestNone.

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Industrial-scale antifungal film incorporated with Cinnamomum cassia oil: enhancing bread quality attributes and evaluation of organoleptic properties

Affiliations

Industrial-scale antifungal film incorporated with Cinnamomum cassia oil: enhancing bread quality attributes and evaluation of organoleptic properties

Authors

Affiliations

Abstract

Conflict of interest statement

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