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. 2025 Apr 23;34(11):2455-2467.
doi: 10.1007/s10068-025-01870-7. eCollection 2025 Jul.

Waste valorization of Rana chensinensis ovum protein isolates versus commercially available proteins isolates from physicochemical, structural, and functional properties to stabilizing Pickering emulsions

Hang Li  1 , Mengyuan Li  1 , Manman Zhang  1 , Ruixue Liu  2 , Nan Li  2 , Yongsheng Wang  2 , Zhihan Wang  2 , Shihan Wang  1
Affiliations

Waste valorization of Rana chensinensis ovum protein isolates versus commercially available proteins isolates from physicochemical, structural, and functional properties to stabilizing Pickering emulsions

Hang Li et al. Food Sci Biotechnol. .

Abstract

This study demonstrated the commercialization potential of Rana chensinensis ovum protein isolates (RCOPI) by comparing it with commercially available proteins from physicochemical, structural, and functional properties to stabilizing Pickering emulsions. The size of RCOPI colloids nanoparticles reached 65 nm which is much smaller than the commercially available proteins. Besides, RCOPI exhibited incredible emulsion ability with the emulsion drop around 22 μm diameters and the zeta potential approximately - 50 mV. The emulsions stabilized by RCOPI showed remarkable stability and the Lactalysis index was about 21% after 100 days storage. In the encapsulation test, scanning electron microscopy (SEM) showed the size of the microcapsule ranging from 20 to 50 μm. The encapsulated efficiency of RCOPI stabilized emulsion was as good as the compared proteins, up to 75%. Furthermore, the results of the sensory evaluation showed that RCOPI stabilized emulsions received the highest approval ratings for appearance, taste, flavor and overall acceptability.

Supplementary information: The online version contains supplementary material available at 10.1007/s10068-025-01870-7.

Keywords: Microencapsulation; Pickering emulsion; Protein; Rana chensinensis ovum; Waste valorization.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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References

    1. Brishti FH, Chay SY, Muhammad K, Ismail-Fitry MR, Zarei M, Karthikeyan S, Saari N. Effects of drying techniques on the physicochemical, functional, thermal, structural and rheological properties of mung bean (Vigna radiata) protein isolate powder. Food Research International. 138: 109783-109783 (2020) - PubMed
    1. Calvez J, Azzout-Marniche D, Tomé D. Protein quality, nutrition and health. Frontiers in Nutrition. 11: 1406618 (2024) - PMC - PubMed
    1. Camargo TR, Khelissa S, Chihib NE, Dumas E, Wang J, Valenti WC, Gharsallaoui A. Preparation and characterization of microcapsules containing antioxidant fish protein hydrolysates: A new use of bycatch in brazil. Marine Biotechnology. 23: 321-330 (2021) - PubMed
    1. Carneiro HCF, Tonon RV, Grosso CRF, Hubinger MD. Encapsulation efficiency and oxidative stability of flaxseed oil microencapsulated by spray drying using different combinations of wall materials. Journal of Food Engineering. 115: 443-451 (2013)
    1. Chen L, Subirade M. Elaboration and characterization of soy/zein protein microspheres for controlled nutraceutical delivery. Biomacromolecules. 10: 3327-3334 (2009) - PubMed