Complete genome sequence of Staphylococcus aureus strain MD04 isolated from the foot ulcer of a patient with diabetes in rural southwestern Uganda

doi:10.1128/mra.00362-25

. 2025 Jul 10;14(7):e0036225.

doi: 10.1128/mra.00362-25. Epub 2025 Jun 10.

Complete genome sequence of Staphylococcus aureus strain MD04 isolated from the foot ulcer of a patient with diabetes in rural southwestern Uganda

Danladi Makeri¹, Emmanuel Eilu², Martin Odoki^{2

3}, Ismail Abiola Adebayo⁴, Reuben Maghembe^{5

6

7}, Samweli Bahati⁵, Musoba Abubakar⁸, Reagan Muhwezi¹, Theophilus Pius⁹, Priscilla Peter Dilli¹⁰, Saheed Adekunle Akinola¹¹, Ezera Agwu^{1

11}

Affiliations

¹ Department of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
² Department of Microbiology and Immunology, School of Medicine, King Ceasor University, Kampala, Uganda.
³ Department of Applied Sciences, School of Sciences, Nkumba University, Entebbe, Uganda.
⁴ Department of Microbiology, Parasitology, and Immunology, School of Medicine, Kabale University, Kabale, Uganda.
⁵ Omics and Bioinformatics Section, DABA Biotech Ltd, Dar es Salaam, Tanzania.
⁶ Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences, Ifakara, Tanzania.
⁷ Department of Immunology and Molecular Biology, College of Health Sciences, Makerere University, Kampala, Uganda.
⁸ Institute of Biomedical Research, Kampala International University Western Campus, Ishaka, Uganda.
⁹ Department of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, Uganda.
¹⁰ Department of Public Health, Kampala International University Western Campus, Ishaka, Uganda.
¹¹ Department of Microbiology and Parasitology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Butare, Rwanda.

PMID: 40492780
PMCID: PMC12243550
DOI: 10.1128/mra.00362-25

Complete genome sequence of Staphylococcus aureus strain MD04 isolated from the foot ulcer of a patient with diabetes in rural southwestern Uganda

Danladi Makeri et al. Microbiol Resour Announc. 2025.

. 2025 Jul 10;14(7):e0036225.

doi: 10.1128/mra.00362-25. Epub 2025 Jun 10.

Authors

Affiliations

¹ Department of Microbiology and Immunology, Kampala International University Western Campus, Ishaka, Uganda.
² Department of Microbiology and Immunology, School of Medicine, King Ceasor University, Kampala, Uganda.
³ Department of Applied Sciences, School of Sciences, Nkumba University, Entebbe, Uganda.
⁴ Department of Microbiology, Parasitology, and Immunology, School of Medicine, Kabale University, Kabale, Uganda.
⁵ Omics and Bioinformatics Section, DABA Biotech Ltd, Dar es Salaam, Tanzania.
⁶ Department of Microbiology and Parasitology, Faculty of Medicine, St. Francis University College of Health and Allied Sciences, Ifakara, Tanzania.
⁷ Department of Immunology and Molecular Biology, College of Health Sciences, Makerere University, Kampala, Uganda.
⁸ Institute of Biomedical Research, Kampala International University Western Campus, Ishaka, Uganda.
⁹ Department of Medical Laboratory Science, Kampala International University Western Campus, Ishaka, Uganda.
¹⁰ Department of Public Health, Kampala International University Western Campus, Ishaka, Uganda.
¹¹ Department of Microbiology and Parasitology, School of Medicine and Pharmacy, College of Medicine and Health Sciences, University of Rwanda, Butare, Rwanda.

PMID: 40492780
PMCID: PMC12243550
DOI: 10.1128/mra.00362-25

Abstract

We report the whole-genome sequence of Staphylococcus aureus strain MD04, isolated from the foot ulcer of a diabetic patient in rural southwestern Uganda. The assembled genome is 2,789,538 base pairs in length, with a GC content of 32.5%. Genome completeness is 98%, with a 2.48% contamination.

Keywords: Illumina sequencing; Uganda; WGS; bacterial genomics; diabetic foot infection; diabetic foot ulcers; wound bacteria.

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Conflict of interest statement

The authors declare no conflict of interest.

References

1. Abbas ZG, Boulton AJM. 2022. Diabetic foot ulcer disease in African continent: “From clinical care to implementation” - Review of diabetic foot in last 60 years - 1960 to 2020. Diabetes Res Clin Pract 183:109155. doi: 10.1016/j.diabres.2021.109155 - DOI - PubMed
1. Rigato M, Pizzol D, Tiago A, Putoto G, Avogaro A, Fadini GP. 2018. Characteristics, prevalence, and outcomes of diabetic foot ulcers in Africa. a systemic review and meta-analysis. Diabetes Res Clin Pract 142:63–73. doi: 10.1016/j.diabres.2018.05.016 - DOI - PubMed
1. Makeri D, Eilu E, Odoki M, Agwu E. 2024. A systematic review of the microbial landscape of diabetic foot ulcers in Uganda. Infect Drug Resist 17:143–151. doi: 10.2147/IDR.S446838 - DOI - PMC - PubMed
1. Makeri D, Odoki M, Eilu E, Agwu E. 2023. Update on prevalence and antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot ulcers in Africa: a systematic review and meta-analysis. Bull Natl Res Cent 47. doi: 10.1186/s42269-023-01119-5 - DOI
1. Macdonald KE, Boeckh S, Stacey HJ, Jones JD. 2021. The microbiology of diabetic foot infections: a meta-analysis. BMC Infect Dis 21:770. doi: 10.1186/s12879-021-06516-7 - DOI - PMC - PubMed

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[1] Abbas ZG, Boulton AJM. 2022. Diabetic foot ulcer disease in African continent: “From clinical care to implementation” - Review of diabetic foot in last 60 years - 1960 to 2020. Diabetes Res Clin Pract 183:109155. doi: 10.1016/j.diabres.2021.109155 - DOI - PubMed

[2] Abbas ZG, Boulton AJM. 2022. Diabetic foot ulcer disease in African continent: “From clinical care to implementation” - Review of diabetic foot in last 60 years - 1960 to 2020. Diabetes Res Clin Pract 183:109155. doi: 10.1016/j.diabres.2021.109155 - DOI - PubMed

[3] Rigato M, Pizzol D, Tiago A, Putoto G, Avogaro A, Fadini GP. 2018. Characteristics, prevalence, and outcomes of diabetic foot ulcers in Africa. a systemic review and meta-analysis. Diabetes Res Clin Pract 142:63–73. doi: 10.1016/j.diabres.2018.05.016 - DOI - PubMed

[4] Rigato M, Pizzol D, Tiago A, Putoto G, Avogaro A, Fadini GP. 2018. Characteristics, prevalence, and outcomes of diabetic foot ulcers in Africa. a systemic review and meta-analysis. Diabetes Res Clin Pract 142:63–73. doi: 10.1016/j.diabres.2018.05.016 - DOI - PubMed

[5] Makeri D, Eilu E, Odoki M, Agwu E. 2024. A systematic review of the microbial landscape of diabetic foot ulcers in Uganda. Infect Drug Resist 17:143–151. doi: 10.2147/IDR.S446838 - DOI - PMC - PubMed

[6] Makeri D, Eilu E, Odoki M, Agwu E. 2024. A systematic review of the microbial landscape of diabetic foot ulcers in Uganda. Infect Drug Resist 17:143–151. doi: 10.2147/IDR.S446838 - DOI - PMC - PubMed

[7] Makeri D, Odoki M, Eilu E, Agwu E. 2023. Update on prevalence and antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot ulcers in Africa: a systematic review and meta-analysis. Bull Natl Res Cent 47. doi: 10.1186/s42269-023-01119-5 - DOI

[8] Makeri D, Odoki M, Eilu E, Agwu E. 2023. Update on prevalence and antimicrobial resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot ulcers in Africa: a systematic review and meta-analysis. Bull Natl Res Cent 47. doi: 10.1186/s42269-023-01119-5 - DOI

[9] Macdonald KE, Boeckh S, Stacey HJ, Jones JD. 2021. The microbiology of diabetic foot infections: a meta-analysis. BMC Infect Dis 21:770. doi: 10.1186/s12879-021-06516-7 - DOI - PMC - PubMed

[10] Macdonald KE, Boeckh S, Stacey HJ, Jones JD. 2021. The microbiology of diabetic foot infections: a meta-analysis. BMC Infect Dis 21:770. doi: 10.1186/s12879-021-06516-7 - DOI - PMC - PubMed

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Complete genome sequence of Staphylococcus aureus strain MD04 isolated from the foot ulcer of a patient with diabetes in rural southwestern Uganda

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Complete genome sequence of Staphylococcus aureus strain MD04 isolated from the foot ulcer of a patient with diabetes in rural southwestern Uganda

Authors

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