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. 2025:2942:177-186.
doi: 10.1007/978-1-0716-4627-4_15.

Mucins and Bacterial Interactions

Yannick Rossez  1 , Renaud Léonard  2 , Olivier Vidal  2 , Zina Chouit  2 , Yann Guerardel  2 , Edwige Madec  2 , Catherine Robbe-Masselot  2
Affiliations

Mucins and Bacterial Interactions

Yannick Rossez et al. Methods Mol Biol. 2025.

Abstract

The handling of high-molecular-weight mucins requires specialized methods. The present manuscript introduces a slot blotting-based technique to screen post-ultracentrifugation fractions using colorimetric assays. Next, we describe an easy slot blot-based method to characterize mucin-bacteria interactions and to assess the effects of glycan enzymatic treatment on bacterial adhesion.

Keywords: Adhesion; Bacteria; Glycans; Mucin; Slot blot.

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