Complete genome sequence of a novel Acinetobacter spp. linked to an outbreak of sepsis following apheresis platelet transfusion

Abstract

During an outbreak investigation of infections following apheresis platelet transfusion, a potentially novel species in the Acinetobacter calcoaceticus-baumannii complex was identified. Here, we report the hybrid Nanopore-Illumina assembly resulting in a complete circular genome sequence of a strain of this species isolated from apheresis platelet product collected during the investigation.

Keywords: Acinetobacter calcoaceticus-baumannii complex; DNA sequencing; bioinformatics; hybrid assembly; outbreak investigation; platelet transfusion.

Fig 1

Phylogenetic analysis places strain 2019-01-05 within the ACBC lineage but distinct from other species. A RAxML maximum likelihood analysis of isolate 2019-01-05 with 351 complete ACBC RefSeq genomes (283 A. baumannii [green], 27 A. pittii [pink], 25 A. seifertii [yellow], 11 A. nosocomialis [purple], 3 A. calcoaceticus [light blue], and 2 A. lactucae [dark blue]) from the concatenated alignment of 969 genes produced by Roary. Branch-level bootstrap support values of 100/100 bootstrap estimates are denoted with a gray circle at the branch node. Genomes color-coded by species obtained from NCBI’s RefSeq database; a red circle denotes placement of the novel ACBC strain on the tree with red branch. An Acinetobacter nosocomialis, GCF_041021905.1, was the closest related reference genome from RefSeq release 229 by FastANI v1.33, with 90.93% average nucleotide identity (ANI) across 85.95% coverage.