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. 2025 May 22;30(11):2278.
doi: 10.3390/molecules30112278.

Quorum Sensing and Mobility Inhibition of Pathogenic Bacteria by Fulvifomes mexicanus sp. nov

Angelica Bolaños-Nuñez  1   2 , Michelle Martínez-Pineda  1 , Ricardo Valenzuela  1 , Mario Figueroa  3 , Albert D Patiño  3 , Everardo Curiel-Quesada  2 , César Ramiro Martínez-Gonzáles  4 , Rodrigo Villanueva-Silva  5 , Tania Raymundo  1 , Abigail Pérez-Valdespino  2
Affiliations

Quorum Sensing and Mobility Inhibition of Pathogenic Bacteria by Fulvifomes mexicanus sp. nov

Angelica Bolaños-Nuñez et al. Molecules. .

Abstract

The development of antimicrobial resistance drives the search for molecules capable of inhibiting bacterial virulence. Fungi of the Basidiomycota phylum constitute an important source of compounds with antimicrobial activity. The present paper describes a new species named Fulvifomes mexicanus sp. nov. based on morphological and phylogenetic analyses. The methanolic extract of basidiome of this fungus inhibited the motility of Pseudomonas aeruginosa ATCC 9027 and the production of violacein by Chromobacterium violaceum CV026. The metabolomic study of the extract by liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC-HRESIMS) and molecular networking analyses revealed the presence of a complex composition of metabolites including hispidin derivatives, terpenoids, phenols, furanones, alkylglycerols, pyrones, and γ-butyrolactones, among others. Overall, this work represents the first chemical and biological study of a new species of Fulvifomes mexicanus as a source of antipathogenic metabolites for the development of novel antimicrobial agents.

Keywords: Fulvifomes mexicanus sp. nov.; antipathogenic activity; metabolic analysis.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Basidiomata of the type specimens examined (A–I) and phylogenetic reconstruction based on the ITS and LSU regions (J). MP and BI analyses recovered identical topologies concerning the relationships among the main clades of Fulvifomes members. For each node, the following values have been provided: MP (≥70%, left)/ML bootstrap (≥70%, middle) and the BI posterior probability (≥0.85, right). The scale bar represents the expected number of nucleotide substitutions per site.
Figure 2
Figure 2
Effect of F. mexicanus sp. nov. methanolic extract on bacterial motility. Panels (A,B) show the growth curves of S. typhimurium ATCC 7251 and P. aeruginosa ATCC 9027, respectively. Panels (C,D) show the motility assay in S. typhimurium ATCC 7251, and (E,F) of P. aeruginosa ATCC 9027. We noted significant difference from control generated by Dunnet’s test, *** p < 0.0001.
Figure 3
Figure 3
(A) Effect of F. mexicanus sp. nov. methanolic extract on growth and (B) specific violacein production of C. violaceum CV026. Significant difference with respect to control was noted (Dunnet’s test), *** p < 0.0001; ** p < 0.01.
Figure 4
Figure 4
Molecular networking analysis of F. mexicanus sp. nov. methanolic extracts with automatic (by GNPS) and manual annotations. Molecular formulas correspond to the observed adduct.
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