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. 2025 May;17(Suppl 1):S269-S271.
doi: 10.4103/jpbs.jpbs_675_25. Epub 2025 Apr 29.

In vitro Antibacterial Efficacy of Various Root Canal Sealers Against Enterococcus Faecalis

Maneesha Das  1 , Prachi Joshi  2 , Arpita Hasmukhlal Jariwala  3 , Sheetal Shivaji Patil  4 , Aditi Singh  5 , Debkant Jena  6
Affiliations

In vitro Antibacterial Efficacy of Various Root Canal Sealers Against Enterococcus Faecalis

Maneesha Das et al. J Pharm Bioallied Sci. 2025 May.

Abstract

Objective: The objective of this in vitro study is to evaluate and compare the antibacterial effectiveness of different root canal sealers against Enterococcus faecalis.

Materials and methods: The antibacterial efficacy of four root canal sealers-three bioceramic materials (TotalFill Bioceramic Sealer, BioRoot Root Canal Sealer, and MTA Fillapex) and one epoxy resin-based sealer (AH Plus)-against E. faecalis (ATCC 29212) was assessed using a modified direct contact test. Every sealer was made under the manufacturer's instructions, and its antibacterial activity was evaluated against newly mixed and set sealers (1 and 3 days) at various intervals (2, 5, 20, and 60 min). Mitis salivarius agar was used to plate the bacterial suspension, and one-way ANOVA was used for statistical analysis.

Results: TotalFill Bioceramic Sealer showed the highest antibacterial efficacy, followed by BioRoot Root Canal Sealer, MTA Fillapex, and AH Plus.

Conclusion: Bioceramic root canal sealers, particularly TotalFill, demonstrate superior antibacterial efficacy against E. faecalis compared to epoxy resin-based sealers such as AH Plus.

Keywords: Antibacterial efficacy; Enterococcus faecalis; endodontic treatment; root canal sealers.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Antibacterial efficacy of various root canal sealers
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