Integrating Lifestyle Modifications and Advanced Sperm Separation Technology: A Case Study on Overcoming Male Infertility Using the LensHooke CA0 Device

Abstract

This study describes the disadvantages of the centrifugation method along with the utilization of high-technology sperm separation to overcome infertility. A 31-year-old female and a 36-year-old male were presented with primary infertility in Maharashtra. The female had normal hormonal profiles; however, the male had a sperm deoxyribonucleic acid (DNA) fragmentation (SDF) index of 46%, which may be due to lifestyle factors such as occupational exposure to chemicals and alcohol use. The contribution to the rise in the SDF was previously due to conventional density gradient centrifugation. To reduce the SDF, the male was put on two months of treatment including lifestyle modification (stress reduction, diet improvement, exercise, etc.) along with the antioxidants. Following this, the sperm was separated through the LensHooke CA0 device that avoids centrifugation. This reduces oxidative stress and DNA damage using a natural motility-based sorting mechanism. Post-treatment, the SDF decreased dramatically to 17%. The couple became pregnant with IUI. This case shows that the LensHooke CA0 device effectively lowers SDF, while ART outcomes improve. These results highlight that lifestyle modification should be implemented along with modern, minimally invasive sperm separation procedures for male infertility treatment.

Keywords: ART; DNA fragmentation; IVF; LensHooke CA0; sperm chromatin dispersion.

Figure 1

LensHooke CA0 device for sperm separation