Complete chloroplast genome sequence data of Cannabis sativa

Abstract

Cannabis sativa, which is an annual erect herb belonging to the Cannabis family, possesses significant medicinal value. The entire genome of the cannabis chloroplast was sequenced using the Illumina NovaSeq 6000 on the Illumina high-throughput sequencing platform. The circular genome has a size of 153,871 base pairs (bp), consisting of two inverted repeats (IRs) that are 26,010 bp each, a large single copy region (LSC) measuring 84,038 bp, and a small single copy region (SSC) of 17,813 bp. A sum of 86 genes were identified and annotated. Among them, there were 48 protein-coding genes (PCGs), 30 tRNA genes, and 8 rRNA genes. Relying on the entire chloroplast genome and the PCGs sequence, a phylogenetic tree of six cannabis plants was established, with Arabidopsis serving as the outgroup.The phylogenetic results showed that Humulus scandens of Cannabaceae was closely related to the hemp in this study. These data enriched the genetic knowledge of Cannabidae plants and provided a basis for further research on molecular breeding and genetic breeding of Cannabidae.

Keywords: Cannabis sativa; Chloroplast genome; Phylogenetic tree.

Fig. 1

Morphology and habitat map of the Cannabis sativa (by Jiao Xu from Pharmacy of college, Heilongjiang University of Chinese Medicine in Harbin, Heilongjiang Province) (126° 40′ 2″ E, 45° 39′ 44″ N).

Fig. 2

Genetic map of the complete chloroplast genome of Cannabis sativa.

Fig. 3

Six Nucleotides and Maximum likelihood (ML) tree based on the complete chloroplast genome sequences of 6 species, include Morus nigra [7], Morus alba [8], Morus cathayana [9], Humulus scandens, these species data are downloaded from the NCBI database (https://www.ncbi.nlm.nih.gov/), (These accession numbers were downloaded from the NCBI website directly). The numbers on the branches are bootstrap values.