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. 2025 May 29:2025:10.17912/micropub.biology.001488.
doi: 10.17912/micropub.biology.001488. eCollection 2025.

Genome Sequence of the Cluster EF Bacteriophage TinyMiny, Isolated using Microbacterium foliorum

Iain Duffy  1 , Juan Jimenez  1 , Luis Gomez  1 , Alexa Keeler  1 , Aisha Ambrose  1 , Moises Guadarrama  1 , Hailey Kerns  1 , Rachael Martin  1 , Jade Moorehead  1 , Arrianne Orgill  1 , Brianna Reiber  1 , Aisha Severe  1 , Leslie Sivley  1 , Genesis Valentin  1 , John Duncan  1
Affiliations

Genome Sequence of the Cluster EF Bacteriophage TinyMiny, Isolated using Microbacterium foliorum

Iain Duffy et al. MicroPubl Biol. .

Abstract

The bacteriophage TinyMiny was isolated from a soil sample collected on the campus of the University of Maryland Baltimore County, in Baltimore, Maryland, using the bacterium Microbacterium foliorum NRRL B-24224. TinyMiny is presumed to be a lytic bacteriophage, consisting of a double-stranded DNA genome 57,042 base pairs in length and encoding 86 genes. Based on gene content similarity to actinobacteriophages, TinyMiny is assigned to the EF cluster.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Virion Morphology of bacteriophage TinyMiny
Figure 1. Virion Morphology of bacteriophage TinyMiny
A negatively stained (1% uranyl acetate) transmission electron micrograph of TinyMiny reveals a siphoviral morphology with an icosahedral capsid and a flexible tail. Scale bar is 100 nm.
See this image and copyright information in PMC

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