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. 1985 Aug 1;229(3):605-9.
doi: 10.1042/bj2290605.

Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A

M Vilanova, J Vendrell, M T López, C M Cuchillo, F X Avilés

Preparative isolation of the two forms of pig pancreatic pro-(carboxypeptidase A) and their monomeric carboxypeptidases A

M Vilanova et al. Biochem J. .

Abstract

A method is reported for the preparative isolation of the two forms of pro-(carboxypeptidase A) from pig pancreas: the monomer and the binary complex with pro-(proteinase E). This method, which is mainly based on chromatography on DEAE-Sepharose at pH 5.7, allows these proenzymes to be prepared more quickly and in safer conditions than with other reported methods. Undegraded and homogeneous carboxypeptidase A1 and A2 species (peptidyl-L-amino acid hydrolase, EC 3.4.17.1), in monomeric forms with high specific activity, are also obtained in high yield by controlled trypsin activation of either of the pro-(carboxypeptidases A) followed by chromatography on DEAE-Sepharose at pH 5.8 under dissociating conditions (7 M-urea).

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References

    1. Biochim Biophys Acta. 1969 Feb 4;175(1):82-96 - PubMed
    1. J Biol Chem. 1965 Jan;240:181-92 - PubMed
    1. J Biol Chem. 1975 Jul 25;250(14):5375-85 - PubMed
    1. J Biol Chem. 1978 Aug 10;253(15):5526-30 - PubMed
    1. J Mol Biol. 1979 Apr 5;129(2):235-64 - PubMed

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