Dysregulated miR-30e-5p in periodontitis inhibits the osteogenic differentiation of human periodontal ligament stem cells by inhibiting CHD7

Abstract

This study is expected to investigate the role of miR-30e-5p in chronic periodontitis (CP) and its diagnostic value in CP. First, the level of miR-30e-5p in CP patients was analyzed, and its ability in the clinical diagnosis of CP was analyzed by Receiver Operating Characteristic (ROC) curve. Subsequently, osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) was induced in vitro. Then, the effect of miR-30e-5p on cell proliferation, apoptosis, ALP activity and osteogenic differentiation markers were analyzed by CCK-8, flow cytometry, commercial kit and RT-qPCR. To investigate whether miR-30e-5p and chromosome helicase DNA binding protein 7 (CHD7) have a targeted relationship, we performed double luciferase reporting and RNA immunoprecipitation assays. Finally, the mechanism of miR-30e-5p and CHD7 jointly regulating osteogenic differentiation of hPDLSCs was investigated in vitro. Compared with healthy controls, the periodontal status of CP patients was worse and the level of miR-30e-5p was higher. The sensitivity and specificity of miR-30e-5p for predicting CP were 76.67% and 80.00%. In addition, the level of miR-30e-5p gradually decreased during the induction of osteogenic differentiation. Inhibiting the expression of miR-30e-5p increased the cell viability of hPDLSCs, and increased the activity of ALP and the level of osteogenic differentiation markers. However, the downregulation of CHD7 reversed a series of results associated with the downregulation of miR-30e-5p. In periodontitis, miR-30e-5p may impede osteogenic differentiation of hPDLSCs by regulating CHD7. Moreover, miR-30e-5p may be a diagnostic biomarker for CP.

Keywords: CHD7; Osteogenic differentiation; Periodontal ligament stem cells; Periodontitis; miR-30e-5p.