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. 1985 Oct;4(10):2461-7.
doi: 10.1002/j.1460-2075.1985.tb03957.x.

LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro

M Vidal, J Sainte-Marie, J R Philippot, A Bienvenue

LDL-mediated targeting of liposomes to leukemic lymphocytes in vitro

M Vidal et al. EMBO J. 1985 Oct.

Abstract

We describe a method for the covalent coupling of low-density lipoproteins (LDL) to the surface of small unilamellar vesicles, and the delivery of the liposome content to leukemic L2C lymphocytes in vitro. We demonstrate the stability of the linkage between LDL and liposomes, the preservation of vesicle integrity and the affinity of the LDL for their specific receptors after the coupling reaction. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted liposomes, and delivered into the cytoplasm of leukemic L2C lymphocytes by the LDL pathway, as demonstrated by the lethal effect on cells measured by 51chromium-release assay.

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