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. 2025 Jun 21;37(4-6):23.
doi: 10.1007/s44445-025-00028-9.

Effect of psidium guajava fruit suspension on bone turnover biomarkers following orthodontic tooth movement in wistar rats

Arwa Aldeeri  1 , Najla Bin Shwish  2 , Rita Khounganian  3 , Abdullah Aldrees  4
Affiliations

Effect of psidium guajava fruit suspension on bone turnover biomarkers following orthodontic tooth movement in wistar rats

Arwa Aldeeri et al. Saudi Dent J. .

Abstract

Bone turnover biomarker levels serve as valid tools for assessing bone resorption and formation. While the standard retention protocol consists of using fixed retainers, removable retainers, or both, attempts have been made to explore alternative approaches using biological agents. Psidium guajava fruit is reported to possess osteoanabolic properties. These properties might be useful in preventing orthodontic treatment relapse. To assess the effect of orally administered Psidium guajava fruit suspension on alkaline phosphatase (ALP) and osteocalcin (OC) levels in the gingival crevicular fluid (GCF) of Wistar rats using enzyme-linked immunosorbent assay (ELISA). Fourteen rats underwent orthodontic tooth movement of the left maxillary first molar. Subsequently, the appliance was removed, then retention phase was initiated by placing composite resin in the created space between the left maxillary first and second molars. The intervention group received 250 mg/kg of P. guajava fruit suspension daily, while the control group received normal saline for 35 days. When retention ended, the left maxillary first molars were allowed to relapse for 7 days. At the end of the relapse phase, GCF samples were collected from the distal side of the left maxillary first molars using a PerioPaper and analyzed by ELISA. The mean concentration of ALP in the control and intervention groups was 0.074 ± 0.012 pg/mL and 0.075 ± 0.005 pg/mL, respectively (P = 0.704), while that of OC was 0.696 ± 0.003 pg/mL and 0.692 ± 0.011 pg/mL, respectively (P = 0.391). Oral supplementation of P. guajava fruit suspension did not have a positive impact on GCF bone turnover biomarkers (ALP and OC) in Wistar rats.

Keywords: Alkaline phosphatase biomarkers; GCF; Orthodontic retention; Orthodontic tooth movement; Osteocalcin; Psidium guajava; Relapse; Wistar rats.

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Conflict of interest statement

Declarations. Ethics approval: All procedures in this study were approved by the Institutional Review Board (#KSU-SE-23–55) and registered at the College of Dentistry Research Center (CDRC) of King Saud University (PR 0162). Ethical approval was obtained from the Research Ethics Committee (REC) office at King Saud University (KSU-SE-23–55). Consent of publication: Not Applicable. Consent of participation: Not Applicable. Conflict of interests: The authors declare no conflict of interests. Institutional review board statement: All procedures in this study were approved by the Institutional Review Board (KSU-SE-23–55), and registered at the College of Dentistry Research Center (CDRC) of King Saud University (PR 0162). Ethical approval was obtained from the Research Ethics Committee (REC) office at King Saud University (KSU-SE-23–55).

Figures

Fig. 1
Fig. 1
Comparison of ALP levels between the control and intervention groups
Fig. 2
Fig. 2
Comparison of OC levels between the control and intervention groups
Fig. 3
Fig. 3
CONSORT flow diagram
See this image and copyright information in PMC

References

    1. Alhadlaq AM (2015) Biomarkers of orthodontic tooth movement in gingival crevicular fluid: A systematic review. J Contemp Dent Pract 16:578–587 - PubMed
    1. Al-Hamdany AK, Al-Khatib AR, Al-Sadi HI (2017) Influence of olive oil on alveolar bone response during orthodontic retention period: Rabbit model study. Acta Odontol Scand 75:413–422 - PubMed
    1. Alhasyimi AA, Rosyida NF, Rihadini MS (2019) Postorthodontic relapse prevention by administration of grape seed (Vitis vinifera) extract containing cyanidine in rats. Eur J Dent 13:629–634 - PMC - PubMed
    1. Ashizawa Y, Sahara N (1998) Quantitative evaluation of newly formed bone in the alveolar wall surrounding the root during the initial stage of experimental tooth movement in the rat. Arch Oral Biol 43:473–484 - PubMed
    1. Bibi T, Khurshid Z, Rehman A, Imran E, Srivastava KC, Shrivastava D (2021) Gingival crevicular fluid (GCF): A diagnostic tool for the detection of periodontal health and diseases. Molecules 26:1–16 - PMC - PubMed

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