Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Sep;104(9):105358.
doi: 10.1016/j.psj.2025.105358. Epub 2025 Jun 2.

Serum amyloid a production in chicken embryonic synovial fibroblasts induced by lipopolysaccharide, interleukin-1β, and vitamin A

Belma Dayı  1 , Alper Sevimli  2 , Ahmet Akkoç  3 , Ayşe Meriç Mutlu  3 , Nurhan Doğan  4
Affiliations

Serum amyloid a production in chicken embryonic synovial fibroblasts induced by lipopolysaccharide, interleukin-1β, and vitamin A

Belma Dayı et al. Poult Sci. 2025 Sep.

Abstract

Amyloid arthropathy, which negatively affects animal welfare by causing various health problems in brown laying hens, is a pathological phenomenon that occurs when amyloid A (AA) protein accumulates in the leg joints and serum amyloid A (SAA), the precursor of AA protein, forms permanent amyloid fibrils. This study aimed to evaluate the SAA production at the 24th and 48th h by enzyme-linked immunosorbent antibody assay (ELISA) in culture medium and real-time quantitative polymerase chain reaction (qPCR) after the induction of chicken embryonic synovial fibroblasts (CESF) with different doses of lipopolysaccharide (LPS), interleukin-1β (IL-1β) and vitamin A. Hemacolor staining, immunocytochemistry, and carbon powder uptake test were performed to characterize the isolated CESF in the study. A statistically significant increase was observed in SAA mRNA expression in all groups at the 24th and 48th h, except the group induced with vitamin A for 48 h, while no increase was detected in the SAA level by ELISA. In the qPCR results, the highest increase among the 24-hour groups was 2604.5 ± 476 in the LPS (10 μg/mL) + IL-1β (30 ng/mL) applied group, while the highest increase among the 48-hour groups was 1577.4 ± 326.4 in the LPS (5 μg/mL) + IL-1β (50 ng/mL) + Vitamin A applied group. The results have shown that SAA expression in CESF is particularly dependent on LPS concentration, with IL-1β and vitamin A being less effective CESF from the brown layer hen embryos proved to be a suitable study model for amyloid arthropathy.

Keywords: Chicken embryonic synovial fibroblast; Interleukin-1β; Serum amyloid A; Vitamin A, qPCR.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig 1
Cell growths from synovial tissue explant (E), inverted microscope. (a) Cell proliferation on the 3rd day. (b) Cell proliferation on the 4th day. (c) Cell proliferation on the 5th day.
Fig 2
Fig 2
Characterization of cells. (a) Primary synovial fibroblasts, vimentin-positive immunostaining, DAB, x200. (b) Primary synovial fibroblasts, cytokeratin-negative immunostaining, DAB, x100. (c) Primary synovial fibroblasts, hemacolor staining, x100. (d) Carbon powder uptake test, non-phagocytosed carbon particles among primary synovial fibroblasts, inverted microscope.
Fig 3
Fig 3
SAA mRNA expression at 24 h groups. *p < 0.001, (y axis shown logarithmically).
Fig 4
Fig 4
SAA mRNA expression at 48 h groups. *p < 0.001, (y axis shown logarithmically).
See this image and copyright information in PMC

References

    1. Abdelhamid L., Hussein H., Ghanem M., Eissa N. Retinoic acid-mediated anti-inflammatory responses in equine immune cells stimulated by LPS and allogeneic mesenchymal stem cells. Res. Vet. Sci. 2017;114:225–232. - PubMed
    1. Akkoc A., Kahraman M.M. Matrix metalloproteinase expression in primary lung fibroblasts of layer type chickens. Biotech. Histochem. 2012;87:201–207. - PubMed
    1. Bartok B., Firestein G.S. Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis. Immunol. Rev. 2010;233:233–255. - PMC - PubMed
    1. Blanco A.E., Barz M., Icken W., Cavero D., Mazaheri A., Voss M., Schmutz M., Preisinger R. Twenty years of amyloid arthropathy research in chickens. Worlds. Poult. Sci. J. 2016;72:495–508.
    1. Bochsler P.N., Slauson D.O. In: Inflammation and Repair of Tissue. Pages 223-225 in Mechanism of Disease. A Textbook of Comparative General Pathology. Slauson D.O., Cooper B.J., editors. Mosby; St Louis, USA: 2002.

MeSH terms