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. 2025 Jun;28(6):543-548.
doi: 10.4103/JCDE.JCDE_213_25. Epub 2025 Jun 2.

Change in serum biomarker levels in patients of apical periodontitis after root canal treatment using two different root canal sealers: A randomized controlled trial

Jyoti Sharma  1 , Pravin Kumar  1 , Shailja Sharma  2 , Vinay Kumar Chugh  3 , Sharmila Shanmugam  1 , Arun Kumar Patnana  4
Affiliations

Change in serum biomarker levels in patients of apical periodontitis after root canal treatment using two different root canal sealers: A randomized controlled trial

Jyoti Sharma et al. J Conserv Dent Endod. 2025 Jun.

Abstract

Aim: The aim of the study was to comparatively evaluate the difference in serum pro-inflammatory (soluble cluster of differentiation-14 [sCD14]) and anti-inflammatory (interleukin-10 [IL-10]) biomarkers levels when 2 different root canal sealers are used during root canal treatment (RCT) in systemically healthy patients with apical periodontitis.

Methods: The study was a parallel double-blind randomized clinical trial; 54 carious mature permanent teeth with apical periodontitis were randomized using a block randomization technique to either Group 1 (Tubli-seal) (n = 27) or Group 2 (BioRoot RCS) (n = 27). Participants received root canal treatment (RCT) and were followed up at 15 days and 2 months. Venous blood samples were obtained, and serum pro-inflammatory, sCD14, and anti-inflammatory, IL-10 biomarkers were assessed by ELISA, and Periapical healing was evaluated by periapical index (PAI). Intragroup and intergroup analysis was done using the Friedman and Mann-Whitney U-test, respectively.

Results: In Group 1, pro-inflammatory biomarker (sCD-14) demonstrated statistically significant results (P = 0.008). Regarding anti-inflammatory biomarker (IL-10), neither of the groups displayed statistically significant changes (P > 0.05). An analysis of the PAI scores revealed statistically significant improvements from preoperative to postoperative levels for both groups (P = 0.00).

Conclusion: RCT reduces serum pro-inflammatory biomarker levels and no significant changes in levels of anti-inflammatory biomarkers in patients of apical periodontitis.

Keywords: Apical periodontitis; BioRoot RCS sealer; root canal treatment; serum biomarkers.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
CONSORT flow diagram
Figure 2
Figure 2
Line diagram showing biomarkers’ mean rank over different time intervals: (a). Tubli-seal group (b) BioRoot RCS group
See this image and copyright information in PMC

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