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. 2025 Jun 2:10:303.
doi: 10.12688/wellcomeopenres.24309.1. eCollection 2025.

The genome sequence of a soldier fly, Nemotelus pantherinus (Linnaeus, 1758)

Anthony Irwin  1 , Ryan Mitchell  2 , Olga Sivell  3 ; Natural History Museum Genome Acquisition Lab; Darwin Tree of Life Barcoding collective; Wellcome Sanger Institute Tree of Life Management, Samples and Laboratory team; Wellcome Sanger Institute Scientific Operations: Sequencing Operations; Wellcome Sanger Institute Tree of Life Core Informatics team; Tree of Life Core Informatics collective; Darwin Tree of Life Consortium
Affiliations

The genome sequence of a soldier fly, Nemotelus pantherinus (Linnaeus, 1758)

Anthony Irwin et al. Wellcome Open Res. .

Abstract

We present a genome assembly from a male specimen of Nemotelus pantherinus (soldierfly; Arthropoda; Insecta; Diptera; Stratiomyidae). The genome sequence has a total length of 804.69 megabases. Most of the assembly (95.93%) is scaffolded into 6 chromosomal pseudomolecules, including the X and Y sex chromosomes. The mitochondrial genome has also been assembled, with a length of 19.1 kilobases. Gene annotation of this assembly on Ensembl identified 27,389 protein-coding genes.

Keywords: Diptera; Nemotelus pantherinus; chromosomal; genome sequence; soldierfly.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Photograph of the Nemotelus pantherinus (idNemPant2) specimen used for genome sequencing.
Figure 2.
Figure 2.. Genome assembly of Nemotelus pantherinus, idNemPant2.1: metrics.
The BlobToolKit snail plot provides an overview of assembly metrics and BUSCO gene completeness. The circumference represents the length of the whole genome sequence, and the main plot is divided into 1,000 bins around the circumference. The outermost blue tracks display the distribution of GC, AT, and N percentages across the bins. Scaffolds are arranged clockwise from longest to shortest and are depicted in dark grey. The longest scaffold is indicated by the red arc, and the deeper orange and pale orange arcs represent the N50 and N90 lengths. A light grey spiral at the centre shows the cumulative scaffold count on a logarithmic scale. A summary of complete, fragmented, duplicated, and missing BUSCO genes in the diptera_odb10 set is presented at the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963978885.1/dataset/GCA_963978885.1/snail.
Figure 3.
Figure 3.. Genome assembly of Nemotelus pantherinus, idNemPant2.1: BlobToolKit GC-coverage plot.
Blob plot showing sequence coverage (vertical axis) and GC content (horizontal axis). The circles represent scaffolds, with the size proportional to scaffold length and the colour representing phylum membership. The histograms along the axes display the total length of sequences distributed across different levels of coverage and GC content. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963978885.1/dataset/GCA_963978885.1/blob.
Figure 4.
Figure 4.. Genome assembly of Nemotelus pantherinus, idNemPant2.1: BlobToolKit cumulative sequence plot.
The grey line shows cumulative length for all scaffolds. Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/GCA_963978885.1/dataset/GCA_963978885.1/cumulative.
Figure 5.
Figure 5.. Genome assembly of Nemotelus pantherinus: Hi-C contact map of the idNemPant2.1 assembly, generated using PretextSnapshot.
See this image and copyright information in PMC

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