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. 2025 Jun;22(227):20240826.
doi: 10.1098/rsif.2024.0826. Epub 2025 Jun 25.

Assessment of diagenesis in archaeological human second metacarpal bones using the intensity of the small angle X-ray scattering D-period peak

Laura Lynne Gardner  1 , William Abramovich  2 , Misha Romanov  2 , Stefan Flohr  3 , Uwe Kierdorf  3 , Horst Kierdorf  3 , Jonathan D Almer  4 , Steven D Jacobsen  5 , Gabriela B Gonzalez  2 , J-S Park  6 , Stuart R Stock  7
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Assessment of diagenesis in archaeological human second metacarpal bones using the intensity of the small angle X-ray scattering D-period peak

Laura Lynne Gardner et al. J R Soc Interface. 2025 Jun.

Abstract

Bone consists mainly of carbonated apatite (cAp) nanoplatelets embedded in a matrix of collagen fibrils. Earlier, high-energy small angle X-ray scattering (SAXS) studies of archaeological adult human second metacarpal bones (mc2) found collagen D-period peaks with high-intensity ID in specimens in which microcomputed tomography (microCT) showed little diagenesis and ID ~ 0 for specimens where microCT revealed severe diagenesis (Park et al. 2022 Int. J. Osteoarchaeol. 32, 170-181 (doi:10.1002/oa.3053); Stock et al. 2022 Int. J. Osteoarchaeol. 32, 120-131 (doi:10.1002/oa.3049)). The present paper uses SAXS at beamline 1-ID, Advanced Photon Source, Argonne National Laboratory and other techniques to study a set of 10 mc2 from an early Medieval cemetery at Greding, Germany. We hypothesized that non-invasive measurement of ID would provide an accurate and rapid (approx. 6 min/specimen) assessment of diagenesis in archaeological mc2. Results of Raman spectroscopy, laboratory microCT and backscattered electron, reflected light and polarized transmitted light microscopies confirmed the SAXS determinations, but lattice parameter values from X-ray diffraction were uncorrelated with ID value. Age-at-death estimates placed the 10 mc2 in three age categories (young adult, middle adult, old adult): lattice parameters from X-ray diffraction were uncorrelated with age at death. Cross-sectional bone area fraction from microCT dropped noticeably for the older age cohort.

Keywords: Raman spectroscopy; collagen; microcomputed tomography; microscopy; preservation of bone; small angle X-ray scattering.

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Conflict of interest statement

We declare we have no competing interests.

References

    1. Nadler M. 1996. Rettungsgrabungen im Schwarzachtal bei Großhöbing. In Das Archäologische Jahr in Bayern - 1996, pp. 139–143. Stuttgart, Germany: Konrad Theiss.
    1. Park J, Laugesen M, Mays S, Birkedal H, Almer JD, Stock SR. 2022. Intact archeological human bones and age at death studied with transmission x‐ray diffraction and small angle x‐ray scattering. Int. J. Osteoarchaeol. 32, 170–181. ( 10.1002/oa.3053) - DOI
    1. Stock SR, Mays S, Turner‐Walker G, Soriano C. 2022. Microcomputed tomography (laboratory and synchrotron) of intact archeological human second metacarpal bones and age at death. Int. J. Osteoarchaeol. 32, 120–131. ( 10.1002/oa.3049) - DOI
    1. Dobberstein RC, Collins MJ, Craig OE, Taylor G, Penkman KEH, Ritz-Timme S. 2009. Archaeological collagen: why worry about collagen diagenesis? Archaeol. Anthropol. Sci. 1, 31–42. ( 10.1007/s12520-009-0002-7) - DOI
    1. Sponheimer M, Ryder CM, Fewlass H, Smith EK, Pestle WJ, Talamo S. 2019. Saving old bones: a non-destructive method for bone collagen prescreening. Sci. Rep. 9, 13928. ( 10.1038/s41598-019-50443-2) - DOI - PMC - PubMed

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