Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Nov 7;83(2):379-84.
doi: 10.1016/0022-1759(85)90260-1.

A rapid and efficient immunization protocol for production of monoclonal antibodies reactive with autoantigens

R Holmdahl, T Moran, M Andersson

A rapid and efficient immunization protocol for production of monoclonal antibodies reactive with autoantigens

R Holmdahl et al. J Immunol Methods. .

Abstract

A simple, time-saving and efficient immunization method suitable for the production of mouse monoclonal antibody secreting hybridomas is described. Draining lymph nodes isolated 9 days after a primary immunization were used as the source of antibody producing cells. No systemic spread of antibody producing cells or specific antibodies could be detected. The present protocol was employed to generate a panel of collagen type II reactive monoclonal antibodies. Most of the monoclonals so generated were found to be of the IgG class.

PubMed Disclaimer

LinkOut - more resources