Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun 11;26(12):5608.
doi: 10.3390/ijms26125608.

Investigating COX-2 and 5-LOX Enzyme-Related Anti-Inflammatory and Antioxidant Activities and Phytochemical Features of Scutellaria salviifolia Benth

Gülsüm Metkin  1 , İpek Süntar  2 , Fatma Sezer Şenol Deniz  2 , Osman Tugay  3 , Mustafa Demiralp  4 , Valeria Pittalà  5
Affiliations

Investigating COX-2 and 5-LOX Enzyme-Related Anti-Inflammatory and Antioxidant Activities and Phytochemical Features of Scutellaria salviifolia Benth

Gülsüm Metkin et al. Int J Mol Sci. .

Abstract

Scutellaria species are widely utilized and have demonstrated diverse biological effects for various diseases, both globally and in traditional Chinese medicine, due to the presence of bioactive compounds with unique structures. This study was conducted to reveal the in vitro effects and phytochemical properties of Scutellaria salviifolia Benth., an endemic species of Türkiye. The inhibitory effects of methanol extracts prepared separately from the aerial and root parts of S. salviifolia on the COX-2 and 5-LOX enzymes and their DPPH and ABTS radical scavenging activities were evaluated using in vitro methods. Additionally, the phenolic compounds of the extracts were compared based on Q-TOF LC/MS analysis. The extracts of S. salviifolia exhibited a high inhibitory effect on COX-2 enzyme activity, comparable to that of celecoxib. Still, they showed no significant effects in the 5-LOX enzyme inhibition assay. In the antioxidant activity assays, the percentage of inhibitory effects of both extracts against DPPH and ABTS were similar. A total of 29 and 27 compounds were detected in the aerial part and root extracts, respectively. Among the identified compounds, 18 were common to both the aerial part and root extracts. S. salviifolia may serve as a valuable alternative to the most well-known Scutellaria species, including S. baicalensis and S. barbata.

Keywords: 5-LOX; COX-2; Lamiaceae; Scutellaria salviifolia; anti-inflammatory; antioxidant; enzyme inhibition.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
5-LOX and COX-2 enzyme inhibition values of S. salviifolia extracts at 400 µg/mL final concentration (* Zileuton for 5-LOX and celecoxib for COX-2, ns: not significant, **** p < 0.0001).
Figure 2
Figure 2
Total ion chromatogram (TIC) of S. salviifolia aerial part extract in negative (above) and positive (below) ionization modes.
Figure 3
Figure 3
Total ion chromatogram (TIC) of S. salviifolia root extract in negative (above) and positive (below) ionization modes.
See this image and copyright information in PMC

References

    1. Acar M., Taşar N., Akbulut G.B. Anatomical, Micromorphological, Karyological and Biochemical study of Scutellaria orientalis subsp. virens and Scutellaria salviifolia. Kahramanmaraş Sütçü İmam Univ. Tarım Doğa Derg. 2022;25:125–136. doi: 10.18016/ksutarimdoga.vi.970571. - DOI
    1. Shang X., He X., He X., Li M., Zhang R., Fan P., Zhang Q., Jia Z. The Genus Scutellaria: An Ethnopharmacological and Phytochemical Review. J. Ethnopharmacol. 2010;128:279–313. doi: 10.1016/j.jep.2010.01.006. - DOI - PubMed
    1. Yılmaz G., Çiçek M., Demirci B., Başer K.H.C. Essential Oil Compositions of Subspecies of Scutellaria brevibracteata Stapf from Turkey. J. Essent. Oil Res. 2019;31:255–262. doi: 10.1080/10412905.2019.1579762. - DOI
    1. Marsh Z., Yang T., Nopo-Olazabal L., Wu S., Ingle T., Joshee N., Medina-Bolivar F. Effect of Light, Methyl Jasmonate and Cyclodextrin on Production of Phenolic Compounds in Hairy Root Cultures of Scutellaria lateriflora. Phytochemistry. 2014;107:50–60. doi: 10.1016/j.phytochem.2014.08.020. - DOI - PubMed
    1. Grzegorczyk-Karolak I., Gołąb K., Gburek J., Wysokińska H., Matkowski A. Inhibition of Advanced Glycation End-Product Formation and Antioxidant Activity by Extracts and Polyphenols from Scutellaria alpina L. and S. altissima L. Molecules. 2016;21:739. doi: 10.3390/molecules21060739. - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources